Delivery and commissioning of a confocal laser scanning microscope for fast 4D detection.

- 1 piece confocal laser scanning microscope for fast 4D detection

The inverted confocal microscope is designed to investigate very fast in vivo processes during the germ cell development of C. elegans as well as interactions between pathogenic bacteria and their plant hosts. Due to the heterogeneity and internal dynamics of the RNA-protein complexes to be studied, the system should go well beyond the resolution of standard confocal microscopy and reach super resolution level. The thermal and photosensitivity of the biological system and the susceptibility to bleaching of the fluorescence fusion proteins to be used represent special challenges. At least 3 fusion proteins must be present simultaneously in different fluorescence channels over the entire duration of several cell divisions in high resolution, with reference to recognizable embryonic development steps through simultaneous recording of morphometric parameters of cell division be traceable via a transmitted light detector. The confocal laser scanning microscope must allow maximum suppression of the disruptive autofluorescence of plant organelles by freely selectable, highly efficient and narrow laser lines and must be FRET / FLIM capable.