The subject of the order are successive supplies of chemical reagents for the needs of the Institute of Horticulture in Skierniewice. The contracting authority allows partial bids. A detailed description of the subject of the contract is included in Annex 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 1.10, 1.11, 1.12, 1.13, 1.14, 1.15, 1.16, 1.17, 1.18, 1.19, 1.20, 1.21, 1.22, 1.23, 1.24, 1.25, 1.26, 1.27, 1.28, 1.29, 1.30, 1.31, 1.32, 1.33, 1.34, 1.35, 1.36, 1.37, 1.38, 1.39, 1.40, 1.41, 1.42, 1.43, 1.44, 1.45, 1.46, 1.47, 1.48, 1.49, 1.50, 1.51 to the Terms of Reference "Offer form" (respectively for each part of the order).

1 Set to remove inhibitors from DNA preparations, Anti-Inhibitor Kit. Quantity: 250 isolations. The kit should be based on the binding capacity of polyphenolic PCR inhibitors by appropriate absorption particles. The set should include: 250 mini columns and 210 ml absorption particles. DNA particles containing PCR inhibitors should be added to the particles contained in the mini-column, and then centrifuged. After using the kit, the DNA contained in the filtrate should be free of polyphenolic compounds known as potent inhibitors of many enzymatic reactions. The kit should be stored at room temperature.

2 Universal Bead-Beat Micro AX Gravity kit (20 μg DNA capacity), with increased efficiency for the isolation of genomic DNA from various materials. Mechanical lysis procedure. Sample size: up to 2 ml bacterial culture, up to 2 ml mold or yeast from liquid cultures and up to 100 mg from solid cultures, up to 100 mg plant tissue, up to 20 mg animal tissue, up to 100 mg feces, up to 200 mg biological environmental sample, up to 50 mg of other biological materials.

3 Genmic Mini, Universal kit for the isolation of genomic DNA from various materials

4 Loading buffer for agarose electrophoresis, six times concentrated Quantity: 5 x 1 ml. 6x loading buffer composition: 20% Ficoll; 0.02% bromphenol blue; 0.02% xylene cyanols FF. Store at +4 about C. Required application: use of 1 μl buffer for each 5 μl of sample volume.

Gentamycin Sulfate, Sterile 50 mg / ml CAS Number 1405-41-0

1 Sabourda agar with chloramphenicol

2 Nutritional broth, composition (per liter of nutrient solution) peptone 5.00, beef extract 2.00, yeast extract 2.00, sodium chloride 4.00, final pH 7.5 ± 0.1 in 25 about C (after sterilization)

3 Standard agar for counting microorganisms, composed of casein peptone 5.0 g, yeast extract 2.5 g, glucose 1.0 g, agar 15.0 g, final pH 7.2 ± 0.2 at 25 about C (after sterilization).

4 Mannitol agar medium

5 King B loose microbiological medium, for differentiating and determining the number of fluorescent bacteria of the genus Pseudomonas. Composition (g / L): peptone 20 g; dipotassium hydrogen phosphate 1.5 g; magnesium sulfate x 7 H2O 1.5 g; 15 g agar; pH 7.2 ± 0.2 after sterilization and cooling to 25oC

6 Medium for rapid detection of lactose fermentation

7 Lactose-gelatin medium

8 Nutrient agar according to ISO 12780

9 Impression plates

10 Bacteriological agar with properties: gel strength 800-950 g / cm3; gelation temperature: 34-36 about C; liquefaction temperature: ≥85 about C; pH 1.5% solution: 6.0-7.5; humidity ≤10%; ash ≤4%.

11 Aminobac - peptone

12 Proteobak - peptone

13 Nutrient Agar

14 Soy Pepton

15 Aminobak - meat peptone

16 Pepton K (casein)

17 Casein Soy Broth (TSB)

18 LB liquid medium

19 RBC agar medium with chloramphenicol composition (calculated per liter of nutrient solution): Pepton 5.00; Glucose 10.00; Dipotassium hydrogen phosphate 1.00; Chloramphenicol 0.10; Bengal rose 0.025; Agar 15.00; Final pH 5.6 ± 0.1 in 25 about C (after sterilization)

1 Polyethylene glycol 8000, (PEG 8000)

2 RNase away Reagent - Rnase removal reagent for RNA isolations.

1 1,2-Propanediol (propylene glycol) di part

2 Dietanolomina cz.d (C4H11O2N)

3 Acetone

4 Ammonium nitrate NH4NO3

5 Ammonium nitrate NH4NO3

6 Chloroform

7 Ethyl alcohol 96% by volume

8 Ethyl alcohol 99.8% by weight

9 Formalin 36-38% DS

10 Fructose

11 glucose

12 Iodine crystalline

13 Citric acid, cz

14 Acetic acid, 80% vol

15 Sulfuric acid 95%, cz

16 Hydrochloric acid 35-38% by weight

17 MgSO4 x 7H2O - magnesium sulfate, part for tissue cultures,

18 MgSO4 x 7H2O - magnesium sulfate, DS

19 Folin and Ciocaulteua reagent

20 Potassium nitrate KNO3

21 Potassium chloride additive

22 KH2PO4 - potassium dihydrogen phosphate, cz

23 Potassium iodide

24 Saccharose

25 Saccharose

26 Sodium chloride NaCl additive

27 di-Sodium hydrogen phosphate 2 • hydrate DS, Disodium hydrogen phosphate dihydrate Na2HPO4 x 2H2O,

28 Sodium acetate 3 hydrate

29 Anhydrous sodium sulphate Na2SO4

30 Sodium thiosulphate 5 hydrate part 1 kg CHPU reagent for biochemical analysis and staining of polyacrylamide gels

31 Sodium carbonate anhydrous pa

32 NaOH (Sodium hydroxide pellets) cz

33 Toluene

34 Ca (NO3) 2 x 4H2O - calcium nitrate

35 Calcium chloride 2 hydrate CaCl2 2 H2O, CAS number 10035-04-8

36 Calcium carbonate CaCO3

37 Hydrochloric acid, analytical weight 0.1 mol / l 0.1N

38 Sodium hydroxide, fixanale, analytical balance 0.1 mol / l

39 Petroleum ether 46/60 cz

40 Isoamyl alcohol

41 Silver nitrate r – r appointed 0.1 mol / l

42 Potassium permanganate - 0.02 N solution

43 Sodium hydroxide appointed 0.1 mol / dm3 anal

44 Zinc sulphate ZnSo4x7H2O 7 hydrate

45 Sodium dihydrogen phosphate NaH2PO4 anhydrous

46 Phenol 5% by weight; CAS: 108-95-2

47 Ammonium chloride NH4Cl; AR; CAS: 12125-02-9

48 Ammonium dihydrogen phosphate (NH4) H2PO4

49 Ammonium phosphate II basic (NH4) 2HPO4

50 Ammonium sulfate (NH4) SO4

51 Glycerin anhydrous

52 K2HPO4 part da dibasic potassium phosphate

53 KOH potassium hydroxide

54 Sodium acid carbonate

55 Potassium phosphate KH2PO4 cz

56 Gelatin Powder

1 HPLC Alpha-Carotene Standard (AS) (solution)

2 HPLC Standard β-carotene (β-carotene)

3 Lycopene HPLC standard, analytical ChromaDex standard

4 Standard Epigallocatechin gallate CAS No. 989-51-5

5 Standard Epicatechin gallate CAS No 1257-08-5

6 Standard Epigallocatechin CAS No 970-74-1

1 L-Arginine

2 6-BENZYLAMINOPURINE (N6-Benzyladenine) C12H11N5 = 225.3; CAS number 1214-39-7

3 Calcium gluconate monohydrate; CAS number 18016-24-5

4 2-iP C10H13N5 = 203.2; CAS number 2365-40-4

5 "Meta-topoline (6- (3-hydroxybenzylamino) purine), molecular weight: C12H11N5O = 241.4

Assay (HPLC)> 99%, CAS Number: 75737-38-1 "

6 "Inositol, Myo-Inositol

C6H12O6 = 180.2, CAS Number 87-89-8 "

7 Ammonium persulfate, CAS: 7727-54-0, analytical quality

8 Urea for molecular biology, quality for electrophoresis

9 RNase A (DNase free) size 50 mg

10 Fe-EDDHA (Ethylenediamine di-2-hydroxyphenyl acetate ferric) (100 g)

11 Murashige & Skoog Medium with vitamins, packaging 220.3 g per 50 L medium

12 α-Naphthylacetic acid (NAA)

1 KCl saturated solution

2 Saturated KCL solution for refilling electrodes, CAS number: 7447-40-7

3 Buffer pH 100 ml for calibration of the meter

4 Buffer pH 100 ml for calibration of the meter

5 Buffer pH 100 ml for calibration of the meter

1 0.5 M EDTA pH 8.0 for molecular biology

2 1 x TE pH 8.0 for molecular biology

3 Basica lEGQT agarose for molecular biology (free from DNases, RNases, endonuclease and ligase inhibitors and proteases), white powder, intended for gel electrophoresis, easy solubility and fast setting, exceptional transparency and low background ensuring good visibility of the bands, low DNA binding .

4 High resolution agarose is characterized by an intermediate melting and gelation point value compared to classical agarose and low melting agarose. Due to the high gel strength parameters, it can be used to separate short DNA and RNA fragments (20 to 800 base pairs in length), ensuring high resolution, excellent transparency and low background color, especially when staining with ethidium bromide and SYBR Green.

5 Ultra-pure dNTPs set at 100 mM (dATP, dCTP, dGTP, dTTP)

6 "Reverse transcription reagent kit - polymerase chain reaction (RT-PCR) for 150 reactions, in a volume of 50 microliters.

The kit must contain: all reagents / components (optimized mixture of enzymes: reverse transcriptase, Taq DNA polymerase and repair polymerase and RNAz inhibitor, the reaction buffer should contain high quality nucleotides and components that inhibit primer attachment to the matrix at lower temperatures, activation of the polymerase after appropriate hot start), required to perform RT-PCR without opening the tube between the RT and PCR stage.

- the kit should be able to detect from 1 fg of total RNA

- the set must be able to generate cDNA transcripts up to 6500 bp long,

- the kit must be capable of amplifying fragments containing a high content of CG vapors "

7 RNase A (DNAZ free)

8 AvaII restriction enzyme

9 DdeI restriction enzyme

HindIII restriction enzyme

11 RsaI restriction enzyme

12 SpeI restriction enzyme

13 "Taq DNA polymerase, recombinant, high purity thermostable, recommended for standard applications requiring DNA synthesis at high temperature with an approximate mass of 94 kDa, isolated from Thermus aquaticus.

The enzyme catalyzes DNA replication at 74 about C. Its half-life is 40 min. at 95 about C.

The enzyme catalyzes the nucleotide polymerization reaction in the 5´-> 3´ direction in the presence of magnesium ions.

It has 5´-> 3´ exonuclease activity.

It has no 3´-> 5´ exonuclease activity.

Adds A at the 3 'ends. It enables obtaining DNA products with a very wide range of sizes, up to 10 thousand. policy pairs. "

Due to the limited number of characters in the form, it is not possible to provide a full description. A full description of the object of the contract is included in Annex No. 1.17 to the ToR "Offer form part 17"

1 Anthocyanin standard - delphinidin-3-O-glalactoside

2 Anthocyanin standard - petunidin-3-O-glucoside

3 Anthocyanin standard - peonidin-3-O-rutinoside

4 HPLC Isorhamnetion glucoside standard

5 Delphinidin-3-O-rutinoside anthocyanin standard

6 Anthocyanin callistephin chloride standard (glucoside-3-pelargonidine) analytical standard, standard for HPLC

7 Cyanidin-3-O-arabinoside chloride anthocyanin standard, HPLC standard

8 Malvidin-3-O-glucoside (Oenin chloride) anthocyanin standard

9 Anthocyanin standard Kuromanin chloride (cyanidin-glucoside) analytical standard, standard for HPLC

10 Anthocyanin standard Ideanin chloride (cyanidin-galactoside), analytical standard

11 Anthocyanin standard - peonidin-3-O-glucoside, HPLC standard

12 Malvidin-3-O-galactoside anthocyanin standard

13 Anthocyanin standard delphinidin-3-O-glucoside (Myrtillin chloride)

14 HPLC Hyperoside Standard (Quercetin-3-0-galactoside), analytical standard, HPLC standard

15 HPLC Quercetin standard, analytical standard

16 HPLC Rutin Standard (Quercetin-3-0-rutinoside), analytical standard

17 HPLC Quercitrin Standard (Quercetin-3-0-rhamnoside), analytical standard

18 Isoquercetin HPLC Standard (Quercetin-3-0-glucopyranoside), analytical standard, HPLC standard

19 HPLC Standard Isorhamnetin rutinoside

"Kit for rapid (hour or less) isolation of total RNA (including low molecular weight fraction (microRNA) from plants / fungi up to 50 micrograms;

- high quality RNA isolation should be possible, among others with raspberry and strawberry leaves

- phenol-free isolation with pre-filtration of the extract by centrifugation through a filter column

- the kit must allow high-quality RNA isolation, integrity (no or very low degradation) and differentiation (presence of all RNA fractions)

- isolated RNA should be (practically) DNA free and must be suitable for further processing: cDNA library creation, RT-PCR "

1 Affinity Script QPCR cDNA Synthesis Kit. RNA reverse transcription kit optimized for use of the resulting cDNA in qPCR reactions. A fast protocol, with a cDNA synthesis time of no more than 15 minutes, enabling cDNA product size up to 12 kb in less than 25 minutes. The functional range of RNA is not less than 3 pg to 3 μg. The 'master-mix' format allows reducing pipetting errors. Reverse transcriptase active in a temperature spectrum not narrower than 37 to 55 about C.

2 DNA 1000 kit The kit includes 25 plates and a set of reagents (gel, dye, internal marker, size marker). The kit enables the analysis of 300 DNA samples in the range from 25 to 1000 bp. One plate allows the analysis of 12 DNA samples. Required amount of sample needed for 1μl analysis. Required sensitivity 1ng / μl. Manufacturer's certificate required to certify the authorization of the distributor.

3 DNA 7500 kit The kit includes 25 plates and a set of reagents (gel, dye, internal marker, size marker). The kit enables the analysis of 300 DNA samples in the range from 100 to 7500 bp. One plate allows the analysis of 12 DNA samples. Required amount of sample needed for 1μl analysis. Required sensitivity 1ng / μl. Manufacturer's certificate required to certify the authorization of the distributor.

4 6000 Nano RNA Kit, compatible with the Bioanalyzer 2100 from Agilent Technologies. The kit includes 25 plates and a set of reagents (gel, dye, internal marker, size marker). The kit allows the analysis of 300 RNA samples. One plate allows the analysis of 12 total RNA or mRNA samples. Required sample volume needed for analysis 1μl. Required sensitivity 5 ng / μl.

1 Hamilton verified buffer pH = 3.06 (Certified legal buffer pH = 3.06 (in 25 about C), accuracy +/- 0.02, with temperature table for accurate calibration on the packaging, shelf life of 60 months, in a CalPack container with a capacity of 500 ml, enabling direct electrode calibration, with a certificate confirming the quality of the buffer, issued by an accredited laboratory)

2 Hamilton verified buffer pH = 6.00 (Certified legal buffer pH = 6.0 (in 25 about C), accuracy +/- 0.02, with temperature table for accurate calibration on the packaging, shelf life of 60 months, in a CalPack container with a capacity of 500 ml, enabling direct electrode calibration, with a certificate confirming the quality of the buffer, issued by an accredited laboratory)

3 Hamilton verified buffer pH = 8.00 (verified buffer pH = 8.00 (in 25 about C), accuracy +/- 0.02, with temperature table for accurate calibration on the packaging, shelf life of 60 months, in a CalPack container with a capacity of 500 ml, enabling direct electrode calibration, with a certificate confirming the quality of the buffer, issued by an accredited laboratory)

4 Hamilton verified buffer pH = 4.01 (Certified legal buffer pH = 4.01 (in 25 about C), accuracy +/- 0.02, with temperature table for accurate calibration on the packaging, shelf life 60 months, in a CalPack container with a capacity of 500 ml, enabling direct electrode calibration, with a certificate confirming the quality of the buffer, issued by an accredited laboratory)

5 Hamilton verified buffer pH = 7.00 (verified buffer pH = 7.00 (in 25 about C), accuracy +/- 0.02, with temperature table for accurate calibration on the packaging, shelf life 60 months, in a CalPack container with a capacity of 500 ml, enabling direct electrode calibration, with a certificate confirming the quality of the buffer, issued by an accredited laboratory)

6 Conductivity standard 147 uS / cm (Conductivity standard 147 uS / cm (in 25 about C), accuracy +/- 1%, with a temperature table for accurate calibration on the packaging, shelf life of at least 18 months, with a certificate confirming the quality of the buffer, issued by an accredited laboratory)

7 Conductivity standard 1413 uS / cm (Conductivity standard 1413 uS / cm (in 25 about C), accuracy +/- 1%, with a temperature table for accurate calibration on the packaging, shelf life of at least 18 months, with a certificate confirming the quality of the buffer, issued by an accredited laboratory)

1 Fotmer reagent for nitrite determination

2 Reagent for the photometer for potassium determination

3 Reagent for photometer for nitrate determination

4 Reagent for the photometer for calcium determination

1 "DNA polymerase with buffer I (100 mM Tris-HCl, pH 8.3, 500 mM KCl, 15 mM magnesium chloride, 0.01% (w / v) gelatin). DNA polymerase should be a chemically modified form of polymerase that requires thermal activation. Features this enzyme:

• Automated chemical hot-start of the enzyme for increased specificity, sensitivity and reaction efficiency

• Thermal activation released over time improves sensitivity at low copy numbers

• Suitable for multiplex PCR, which saves time and reagents

• The packaging should contain a 10X PCR buffer containing 15 mM MgCl2.

Hot-start activation

The modified enzyme is delivered in an inactive state. After thermal activation, the modifier is permanently released, contributing to the regeneration of the active enzyme. The resulting hot-start amplification provides greater sensitivity, specificity and performance compared to conventional PCR techniques.

The DNA polymerase can be activated partially or completely in the heating step before PCR or it can be activated slowly in a slow manner during the denaturation steps in thermal PCR cycles. During or without the initial heat activation step, the active enzyme is released slowly during the thermal cycle to adjust its concentration and increase specificity. The yield of a specific product increases because reagents are not wasted in creating undesirable products. Because DNA polymerase is a chemical hot-start enzyme, there is no risk of biological contamination. The package should contain 250 units of DNA polymerase and buffer I (100 mM Tris-HCl, pH 8.3, 500 mM KCl, 15 mM magnesium chloride, 0.01% (w / v) gelatin). "

2 DNA 500 Ladder (25 bp DNA) marker needed to analyze / split DNA samples on a MultiNA device

3 "Thermostable PCR hot start DNA polymerase, which provides increased specificity for Taq DNA polymerase. The kit must be equipped with thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial stage of PCR denaturation, improving product performance.

Taq DNA polymerase has matrix independent end transferase activity that adds 3 'deoxyadenosine to the ends of the product and has 5' → 3 'exonuclease activity. PCR products made using Platinum Taq DNA polymerase can be used in the same downstream applications without protocol modification. It must be designed for DNA amplification from complex genomic, viral and plasmid matrices, as well as in RT-PCR.

"

4 Brazilian Platinium Taq polymerase is a convenient and reliable thermostable PCR thermoplastic polymerase that provides greater specificity than Taq DNA polymerase. The property is conferred by thermolabile monoclonal antibodies that make Taq DNA polymerase inactive until the initial stage of PCR denaturation.

5 SYBR Gold fluorescent reagent pack (10,000 concentration in DMSO)

6 Sequencing kit providing an effective, 5-minute, one-time cloning strategy for direct absorption of PCR products with Taq polymerase amplification into plasmid sequencing vector, pCR ™ 4-TOPO® TA vector with specially designed sequence primer sites that provide more insert sequences and fewer vector sequences with each reaction.

1 Ultra potassium dihydrogen phosphate "Ultrapure Potassium Phosphate monobasis", for liquid chromatography and molecular biology, crystalline, purity 99.9%; CAS: 7778-77-0

2 Anhydrous sodium acetate

3 formic acid; Analyzed

4 "Sulfuric acid 95-97%; ANAlYZED Information on basic physical and chemical properties - 95-97%; Ammonium ion (NH4) <1ppm / Heavy metals (as Pb) <1.0 ppm /

Nitrates (NO3) <0.50 ppm / Combustion residue <4.0 ppm "

5 Hydrochloric acid 37-38% by weight

6 Acetone (AR) ACS

7 "Ethyl alcohol 95%, Analyzed Information on basic physical and chemical properties

Content 95.1 - 96.9% (v / v) 96.3% (v / v)

Evaporation residue <0.0003%; Titratable acids (meg / g) 0.00015

Titratable bases (meg / g) 0.00003; "

8 "N-butyl acetate; ANAlYZED Information on basic physical and chemical properties

Content 98%; Density (g / ml) at 20 about C 0.878-0.881 n-Butanol 0.5%; 0.005% evaporation residue; Water 0.05%; Trace pollutants (in ppm); Heavy metals (as Pb) 0.5; Iron (Fe) 0.5 "

9 Methanol for HPLC; Methanol gradient purity for liquid chromatography

10 Acetonitrile for HPLC, Gradient Grade for UV BAKER HPLC ANALYSED

11 "Ethyl acetate for HPLC; Information on basic physical and chemical properties; Content min. 99.9%; Evaporation residue (in ppm): <0.1

Water (volumetric, using KF) 0.01%; Ultraviolet absorbance :; 400-330 nm <0.01

280nm <0.01; 265nm 0.02; UV cut-off, nm 253; Traces of fluorescent pollution; (based on quinine) ppb :; Emission at 450 nm 1; Max. emissions for 1 "pollutants

12 "Acetic acid for HPLC, Information on basic physical and chemical properties; Content (measured by GC, corrected for water) min. 99.9%

Ultraviolet absorbance: (1 cm path in water): 280 nm <0.01; 350 nm <0.01; 0.0005% evaporation residue; Water (KF) <0.1% "

1 Kit that is a mixture of KAPA Taq DNA polymerase and modified archaeal DNA polymerase (type B) with correction capability. The two enzyme system has been specifically designed to perform efficient and sensitive and long PCR reactions.

2 "Reagent kit for qPCR (2 x 5 ml). Contains unique qPCR optimized polymerase using SYBR Green dye. Polymerase is designed to operate under stringent quantitative PCR (qPCR) conditions in real time. KAPA SYBR DNA Polymerase with appropriate buffer systems, it increases the amplification efficiency of difficult matrices: rich in GC and AT pairs.

The kit contains: twice-concentrated Master-Mix with antibody-dependent Hot-Start polymerase, with fluorescent dye SYBR Green I, MgCl2 (2.5 mM), dNTy and stabilizers. Supplied with 50 x ROX high and 50 x ROX Low (200 μl each) "

1 Conductivity standard 0.05 S / m ((25 about C), with a purity of not less than 99.9%, the period of validity of the standard at least 12 months, the standard together with a certificate confirming its metrological properties, containing PCA accreditation symbols together with reference to the PCA status)

2 Conductivity standard 0.15 S / m ((25 about C), with a purity of not less than 99.9%, the period of validity of the standard at least 12 months, the standard together with a certificate confirming its metrological properties, containing PCA accreditation symbols together with reference to the PCA status)

1 Apple Chlorotic Leafspot Virus ACLSV, negative control for 10 tests

2 Apple Chlorotic Leafspot Virus ACLSV, positive control for 10 tests

3 Apple Chlorotic Leafspot Virus ACLSV (IgG and conjugate set / 500)

4 Cucumber Mosaic Virus CMV, negative control for 10 tests

5 Cucumber Mosaic Virus CMV, positive control for 10 tests

6 Cucumber Mosaic Virus CMV (IgG and conjugate set / 100)

7 Prune Dwarf Virus PDV, negative control for 10 tests

8 Prune Dwarf Virus PDV, positive control for 10 tests

9 Prune Dwarf Virus PDV (IgG and conjugate set / 1000)

10 Prunus Necrotic Ringspot Virus PNRSV, negative control for 10 tests

11 Prunus Necrotic Ringspot Virus PNRSV, positive control for 10 tests

12 Prunus Necrotic Ringspot Virus PRNSV (IgG and conjugate set / 100)

13 Arabis Mosaic Virus ArMV, negative control for 10 tests

14 Arabis Mosaic Virus ArMV, positive control deactivated for 10 tests

15 Arabis Mosaic Virus ArMV (IgG and conjugate set / 100)

16 Apple Mosaic Virus ApMV, negative control for 10 tests

17 Apple Mosaic Virus ApMV, positive control for 10 tests

18 Apple Mosaic Virus ApMV (IgG and conjugate set / 500)

19 Raspberry Bushy Dwarf Virus RBDV, negative control for 10 tests

20 Raspberry Bushy Dwarf Virus RBDV, positive control for 10 tests

21 Raspberry Bushy Dwarf Virus RBDV (IgG and conjugate set / 100)

22 Apple Stem Grooving Virus ASGV, negative control for 10 tests

23 Apple Stem Grooving Virus ASGV, positive control for 10 tests

24 Apple Stem Grooving Virus ASGV (IgG and conjugate set / 500)

25 Cherry Leafroll Virus CLRV b, negative control for 10 tests

26 Cherry Leafroll Virus CLRV b, positive control deactivated for 10 tests

27 Cherry Leafroll Virus (birch isolate) CLRV b (IgG and conjugate set / 500)

28 Raspberry Ringspot Virus RpRSV ch, negative control for 10 tests

29 Raspberry Ringspot Virus RpRSVch, positive control deactivated for 10 tests

30 Raspberry Ringspot Virus (cherry isolate) RpRSV ch (IgG and conjugate set / 100)

31 Raspberry Ringspot Virus RpRSVr, negative control for 10 tests

32 Raspberry Ringspot Virus RpRSVr, positive control deactivated for 10 tests

33 Raspberry Ringspot Virus (raspberry isolate) RpRSV r (IgG conjugate set / 100)

34 Plum pox virus (Sharka) PPV IgG antibody 1000 tests pack = 0.2 ml

35 Plum pox virus (Sharka) PPV conjugate 1000 tests pack = 0.2 ml

36 Plum pox virus (Sharka) PPV positive control 12 tests pack = 2.5 ml

37 Plum pox virus (Sharka) PPV negative control 12 tests pack = 2.5 ml

1 Set for DNA isolation from plants, allows for quick and accurate isolation of DNA from plant samples. Contains two different lysis buffers (Lysis Buffer PL1, based on CTAB lysis method and Lysis Buffer PL2, based on lysis method by SDS), which increases the number of possible applications. The included columns and RNase A allow better cleaning of the lysate. An optimized silica membrane improves DNA binding. The kits are suitable for isolating DNA from plant tissues and cells and from fungi. The resulting DNA can be used in PCR, Southern blot and enzymatic reactions. The kit includes silicone membrane technologies and small centrifugation columns. Sample of plant material for analysis <100mg (wet tissue), <20mg (dry tissue). The volume of products received - 50 bp. - 50 kbp Yield - 1-30 μg (100 mg wet tissue). Purity of DNA preparation A260 / A280 - 1.8–1.9. Isolation time - 30 min / trial. Column binding capacity - 50 μg.

2 Set for DNA isolation from plants (Maxi), allows for quick and accurate isolation of DNA from plant samples. Contains two different lysis buffers (Lysis Buffer PL1, based on CTAB lysis method and Lysis Buffer PL2, based on lysis method by SDS), which increases the number of possible applications. The included columns and RNase A allow better cleaning of the lysate. An optimized silica membrane improves DNA binding. The kits are suitable for isolating DNA from plant tissues and cells and from fungi. Sample of plant material for analysis 1500 mg (wet tissue), 300 mg (dry tissue).

The 3 "kit for the isolation of genomic DNA from different types of soils, sediments and wastewater should contain two alternative lysis buffers and special additives (Enhancer SX) for optimal processing of various soil samples in order to obtain high efficiency and purity of the preparation. It should also contain ceramic beads intended for the most effective destruction of cell walls of Gram-positive and Gram-negative bacteria, archaea, yeast, fungi and algae in soil, sewage and sediments, inhibitors removal columns to eliminate all PCR-DNA inhibitors should be ready to use for PCR without dilution Requirements:

• The set should be based on membrane technology with a silicone membrane and contain mini columns.

• Material samples <500 mg soil, sediment or sewage

• Typical yield 2–10 μg DNA (500 mg soil)

• Elution volume 30-100 μL

• Preparation time 90 min / 10 tests

• 50 μg binding capacity

The kit should allow 250 tests of isolation of total DNA from soil, sewage or sediments, should be useful for obtaining total DNA from microorganisms in soil and sediments, identification and detection of microorganisms in environmental samples. The resulting DNA preparation should be of a quality enabling application of: PCR, real-time PCR, Southern blotting, microarray technology "

1 Set of enzymes for determination of TDF / SDF / IDF fiber Complies with the requirements of the FOSS 1023 apparatus according to AOAC 991.42 (1 pack contains a set of enzymes sufficient for 200 analyzes)

2 Set of standards for TDF / SDF / IDF fiber determination Complies with the requirements of the FOSS 1023 apparatus according to AOAC 991.42 (in the package / set analytes of at least: beta-glucan 1 g; high amylase 10g corn starch; 10g wheat starch; casein 5g; pectin 1 g, larch galactan 1 g)

1 ALOA selective agar for Listeria according to Ottaviani and Agosti (ISO 11290), basis

2 ALOA Chromocoult selective supplement for Listeria

3 Ultra-pure agar, granulated for microbiology, Appearance (color) brown-yellow, Appearance (description) granules, Identity (NIR) test passed, Identity (iodine test) passed, Identity (clotting point) test passed, Identity ( turbidity - test) test passed, Sodium sulfate (600 about C) ≤ 5.0%, loss on drying (105 about C; 4 h) ≤ 10%, Ca (calcium) ≤ 0.1%, Mg (Magnesium) ≤ 0.05%, freezing point 32 - 36 about C, Growth (Escherichia coli ATCC 25922 (WDCM 00013)) test passed, Growth (Streptococcus pyogenes ATCC 21059) test passed, Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)) test passed, Growth (Shigella sonnei ATCC 29930 (WDCM 00127) ) test passed, Growth (Streptococcus agalactiae ATCC 13813) test passed, Growth (Streptococcus equinus DSM 20062) test passed, Growth (Streptococcus pneumoniae ATCC 6301) test passed, Growth (Candida albicans ATCC 10231 (WDCM 00054)) test passed, Physicochemical information : melting point 90 about C pH 6.8 (100 g / l, H₂O, 20 about C) bulk density 550 kg / m3 Solubility 20 g / l slightly soluble. Toxicological information LD 50 oral LD50 Rat 11000 mg / kg

4 Sodium hypochlorite solution

5 BAT agar for isolating Alicyclobacillus acideterrestris (granules)

6 Test strips for analyzing the content of Mg ions in solutions, measuring range 5 - 100 mg / l for the RQflex measuring system

7 Test strips for analyzing the content of Ca ions in solutions, measuring range 5 - 125 mg / l for the RQflex measuring system

8 Test strips for analyzing the NO3 ion content of solutions, measuring range 5 - 225 mg / l for the RQflex measuring system

9 Test strips for analyzing the content of PO4 ions in solutions, measuring range 5 - 120 mg / l for the RQflex measuring system

10 Hydrochloric acid 30% Suprapur

11 Nitric acid 65% Suprapur

12 Refractive index, standard set 2 Toluene / water, referred to Refractive index standard kit 2

13 Ammonia solution (NH4OH 25% for HPLC)

14 Ammonium acetate C2H7NO2 (Ammonium Acetate) for HPLC, CAS: 631-61-8

Propidium iodide C27H34I2N4

16 High Pure PCR Product Purification Kit - a set for purifying PCR products from a sample and from agarose gel, contains buffers (Binding Buffer, Wash Buffer, Elution Buffer) and columns (High Pure Spin Filter Tubes with glass fiber) and elution tubes. It completely removes proteins and DNA less than 100bp. The minimum elution volume is 10uL. 250 reaction pack.

17 4-Nitro blue tetrazolium chloride, crystals

18 Test strips: Ascorbic acid is suitable for quickly measuring levels of natural ascorbic acid (vitamin C) in foods such as fruit juices for the Reflectoquant device

Due to the limited number of characters in the form, it is not possible to provide a full description. A full description of the subject of the contract is included in Annex 1.29 to the ToR "Offer form part 29"

1 Ethidium bromide

2 Formamide deionized to molecular biology

3 Mixture: phenol / chloroform / isoamyl (25: 24: 1), stabilized with 0.1% 8-hydroxyquinoline, pH approx. 5.0, heavy metals max. 0.0005%, stability approx. 12 months.

4 Isoamyl alcohol for molecular biology

5 Anhydrous sodium carbonate, biochemical purity

6 Sodium acetate - solution 3 M (pH 5.2) for molecular biology

7 Tris buffer pH 8.0 (1 M) for molecular biology

8 Acrylamide / bisacrylamide 40% solution, 37.5: 1, for molecular biology

1 "Kit Fastgene for isolation of PCR products from gel.

The FastGene® Gel / PCR Extraction Kit has been designed for DNA extraction from gel and purification of PCR products. DNA fragments purified by FastGene® Gel / PCR Extraction Kit are ready for direct use in further applications such as sequencing, cloning, transformation, digestion, in vitro transcription "

2 "100bp DNA ladder ready to use range: 50-1500 bp, number of bands 17, concentration: 112 μg / ml; Packaging: 5 x 50 μg / 500 μl

Recommended: 5 μl / well

Containing orange G dye as tracking dye.

Source: PCR products and double-stranded DNA digested with appropriate restriction enzymes are extracted with phenol and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.

Stable for at least 12 months at 25 about C. In the case of long-term storage, store at 4 about C or -20 about C. "

Isothermal Mastermix, Gsp SSD polymerase, fluorescent dye. Ready-to-use mixture for isothermal DNA amplification. It contains an optimized composition of the reagents necessary for the reaction, in addition to the DNA matrix and primers. MasterMIX contains a fluorescent dye enabling real-time product detection on the Genie II platform and Real-time PCR devices (FAM channel), as well as quantitative analysis of the amplified product. Analysis of the annealing curve allows to determine the specificity of the reaction. It allows conducting reactions in a closed system (called close-tube system).

1 Tris-Acetate-EDTA (TAE) Buffer 50X. pH 8.3

2 10X Tris-Borate-EDTA (TBE) Buffer (1L)

1 MacConkey agar with sorbitol (loose) composition (per liter of medium): casein hydrolyzate 1.5 g, gelatin hydrolyzate 17 g, meat extract 1.5 g, neutral red 0.03 g, sodium chloride 5 g, violet crystalline 0.001 g, sorbitol 10 g, bile salts 1.5 g, agar 13.5 g, pH 7.1 ± 0.2 after sterilization and cooling to 25 C.

2 Brillant Green Agar (Kauffmann medium), loose (selective medium for the isolation of Salmonella: protein peptone 10.0 g / l; yeast extract 3.0 g / l; lactose 10.0 g / l; sucrose 10.0 g / l ; sodium chloride 5.0 g / l; phenol red 0.08 g / l; brilliant green 0.0125 g / l; agar 12.0 g / l; pH 6.9 ± 0.2 in 25 about C)

3 XLD medium, loose (selective medium for the isolation of Salmonella and Shigella from clinical samples and food: yeast extract 3.0 g / l; L-lysine HCl 5 g / l; xylose 3.75 g / l; lactose 7.5 g / l; sucrose 7.5 g / l; sodium deoxycholate 1.0 g / l; sodium chloride 5.0 g / l; sodium thiosulfate 6.8 g / l; iron ammonium citrate 0.8 g / l; phenol red 0.08 g / l; agar 12.5 g / l; pH 7.4 ± 0.2 at 25 about C)

4 PALCAM agar base, loose (selective, differential diagnostic medium for the detection of Listeria monocytogenes: Columbia Blood Agar Base 39.0 g / l; yeast extract 3.0 g / l; glucose 0.5 g / l; esculin 0.8 g / l; iron ammonium citrate 0.5 g / l; mannitol 10.0 g / l; phenol red 0.08 g / l; lithium chloride 15.0 g / l; pH 7.2 ± 0.2 at 25 about C)

5 Fraser broth base, loose (selective, diagnostic enrichment medium for isolation of Listeria spp. From food and environmental samples: proteose peptone 5.0 g / l; trypton 5.0 g / l; powder "Lab-Lemco" 5.0 g / l; yeast extract 5.0 g / l; sodium chloride 20.0 g / l; disodium hydrogen phosphate 12.0 g / l; potassium dihydrogen phosphate 1.35 g / l; aescululin 1.0 g / l; lithium chloride 3.0 g / l; pH 7.2 ± 0.2 in 25 about C).

6 MRSV Agar (MODIFIED SEMI-SOLID RAPPAPORT VASSILIADIS AGAR: 4.6 g / L enzymatic animal and plant tissue extract; 4.6 g / L acid casein hydrolyzate; 7.3 g / L sodium chloride; potassium dihydrogen phosphate (KH2PO4) 1 , 5 g / l; magnesium chloride anhydrous (MgCl2) 10.9 g / l; green malachite oxalate 0.04 g / l; agar 2.7 g / l; pH 5.2 † (5.1 to 5.4 ) at 25 about C)

1 Acetone FOR HPLC

2 NH4H2PO4, monoammonium phosphate, Ammonium dihydrogenorthophosphate

3 Hexane for HPLC

4 L-ascorbic acid

5 Citric acid cz

6 Sodium metabisulphite sodium

7 Ammonium sulphate pda Chemical formula: (NH4) 2SO4

1 Glacial acetic acid, yr 99.5% czda [64-19-7]

2 Salicylic acid, cz [69-72-7]

3 Gibberellic acid ≥ 90.0% [77-06-5]

4 6-Benzylaminopurine ≥ 98.0% [1214-39-7]

5 Jasmonic acid ≥ 85.0% [77026-92-7]

6 Formic acid - Formic acid puriss, bye, ACS reagent, reag. Ph. Eur., ≥98%; CAS number: 64-18-6

7 Gallic acid monohydrate

8 Citric acid cz

1 DNA 2500 Ladder (pGEM DNA Markers) a marker needed to analyze / split DNA samples on a MultiNA device

2 Marker DNA 1000 (PhiX174DNA / HaeIII) marker needed to analyze / split DNA samples on a MultiNA device

3 Trizma Hydrochloride (Tris-HCL)

4 M-MLV reverse transcriptase (Moloney Murine Leukemia Virus Reverse Transcriptase), RNA-dependent DNA polymerase used for cDNA synthesis, can be used for long mRNA fragments (> 5kb), the enzyme consists of one 71kDa subunit. The enzyme is supplied at a concentration of 200u / μl together with a 5X Reaction Buffer reaction buffer with the composition: 250mM Tris-HCl (pH 8.3 at 25 about C), 375mM KCl, 15mM MgCl2, 50mM DTT. M-MLV reverse transcriptase is inactivated by heating at 70 about C for 10 minutes.

5 "RQ1 RNase-Free Dnase, 1000u

DNase degrades single-stranded or double-stranded DNA

• Forms 3 'hydroxyl oligonucleotides during degradation

• Intended for applications where the maintenance of RNA integrity is critical, e.g. isolation of DNA free RNA, DNA degradation from the RNA transcription system, Nick translation, DNA: protein interactions using DNase I footprintig

• Supplied at a concentration of 1u / μl in 10mM HEPES buffer (pH 7.5 at 25 about C), 50% (v / v) glycerol, 10mM CaCl2, 10mM MgCl2

• Along with DNase, a 10-fold concentrated reaction buffer (400mM Tris-HCl [pH 8.0 at 25 about C], 100mM MgSO4, 10mM CaCl2) and buffer stopping the composition (20mM EGTA [pH 8.0 at 25 about C])

• The enzyme is derived from bovine pancreas

• Parameters covered by quality control: enzyme activity and presence of Rnaz "

6 Rnasin Ribonuclease Inhibitor, 40 U / μl

1 DNA isolation kit from the Dneasy Plant Mini Kit: a set of reagents and columns for the isolation and purification of genomic DNA from species such as tomato, rice, tobacco, gypsophila, sunflower, potato. Sample size <100mg, insulation performance 3-30ug. The kit contains: RNase A (solution), buffers: AP1, AW1, AW2, AE;

2 DNA isolation kit from the Dneasy Plant Mini Kit: a set of reagents and columns for the isolation and purification of genomic DNA from species such as tomato, rice, tobacco, gypsophila, sunflower, potato. Sample size <100mg, insulation performance 3-30ug. The kit contains: RNase A (solution), buffers: AP1, AW1, AW2, AE;

3 Kit for total RNA isolation (RNeasy Plant Mini Kit QIAGEN) from plant tissues and fungi. The set is based on the use of RNA binding silica membranes. Total RNA can be isolated from 100-1 x 107 cells or 10-100 mg tissue. Isolation time up to 30 minutes, elution volume 30-100 μl, efficiency 25-60 μg. The kit includes: 50 individually packed RNA isolation columns, 50 homogenization columns, 50 1.5ml tubes, 50 2ml tubes, 45ml RLT buffer, 45ml RLC buffer, 45ml RW1 buffer, 11ml RPE buffer, 10ml free water from Rnaz. Kit for manual and automatic isolation (electronic version dedicated by the manufacturer).

1 Rotiphorese Gel 40 (19: 1), polyacrylamide, ready to use, a mixture of acrylamide and biscrylamide in a 19: 1 ratio

2 Ammonium peroxydisulphate (APS),> 98%, for electrophoresis (PAGE)

3 Silika orange gel 2-5 mm, with moisture indicator changing color from orange to colorless

1 DNA 2500 Kit IVD kits for DNA fragment analysis on a MultiNa device

2 DNA-500 reagent kit designed for the electrophoresis chip system on the MultiNA MCE-202 device (separation buffer for electrophoretic division, marker solution for electrophoretic separation)

3 DNA-1000 reagent kit designed for the electrophoresis chip system on the MultiNA MCE-202 device (separation buffer for electrophoretic division, marker solution for electrophoretic separation)

4 Cleaning solution - synthetic microchip cleaning kit - RA for Microchip Electrophoresis System for DNA / RNA Analysis MCE-202 MultiNA

5 Quartz, synthetic Microchip type WE-C for the device Microchip Electrophoresis System for DNA / RNA Analysis MCE-202 MultiNA

1 Ammonia 25% by weight

2 D - (+) - Anhydrous glucose, cz

3 Nitric acid 65% vol. Dda, FP

4 Hydrochloric acid 35 - 38% on the analytical basis

5 0.1N hydrochloric acid, standard solution; Molar concentration (20 about C) c (HCl) = 0.1 mol / l +/- 0.2%

6 Methanol for HPLC

7 Potassium chloride, cz

8 2-propanol (Isopropanol) for HPLC, 99.8% purity

9 Buffer solution pH 4.00 +/- 0.05

10 Buffer solution pH 7.00 +/- 0.05

11 Buffer solution pH 5.00 +/- 0.05

12 Buffer solution pH 10.00 +/- 0.05

13 Sodium chloride (NaCl)

14 Anhydrous sodium carbonate, anhydrous, 99.95-100.05%

15 Sodium hydroxide 0.1 mol / l (0.1N) analytical balance, fixanale, CAS: 1310-73-2

16 Sodium hydroxide microgranules (NaOH)

17 Calcium chloride 6 hydrate

18 Ethyl alcohol 96% by volume

19 Acetic acid 99.5% - 99.9% DS

1 Agarose for molecular biology; ≤10% humidity; EEO 0.09-0.13; gel point 36 about C (gel 1.5% ± 1.5 about C); gel strength ≥ 1200g / cm2; sulfate content ≤ 0.15%; free from DNaz and RNaz CAS number 9012-36-6

2 C8-C20 Alkanes Standard (Fluka), Standard mixture of C8-C20 alkanes in hexane concentration ~ 40mg / L each, standard to GC;

3 C21-C40 Alkanes Standard (Fluka), Standard mixture of C21-C40 alkanes in toluene at ~ 40mg / L each, standard to GC;

4 Isorhamnetin, 95% purity, HPLC chromatographic standard; CAS number: 480-19-3

5 HPLC Patulin standard, reference material; CAS: 149-29-1

6 HPLC Avicularin (Quercetin 3-O-α-1-arabinofuranoside) standard, analytical standard, HPLC standard; CAS Number: 572-30-5

7 Galacturonic acid monohydrate; CAS number: 91510-62-2

8 Meta-Phosphoric acid purum, ~ 65% HPO3 basis

9 L-Proline, BioUltra standard, ≥99.5%; CAS number: 147-85-3

10 Propidium iodide C27H34I2N4 CAS: 25535-16-4

11 HPLC Capsanthin analytical standard; CAS Number: 465-42-9

Ninhydrin, ACS reagen, tSynonym: 1,2,3-Indantrione monohydrate, 2,2-Dihydroxy-1,3-indanedione, Trioxohydrindene monohydrate; C9H6O4; CAS Number: 485-47-2

13 Heptane, anhydrus-99%, anhydrous, GC-98.5% purity; CAS number: 142-82-5

14 2-Methoxyethanol anhydrous, purity 99.8%; CAS number: 109-86-4

Isoacetate (2,2,4-trimethylpentane), anhydrous, 99.8% purity; CAS number: 540-84-1

Trolox 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid, 98%; CAS number 53188-07-1

17 dipotassium phosphate; K2HPO4; CAS number: 7758-11-4

18 Ellagic Acid HPLC Standard

19 Rifampicin; 3- (4-Methylpiperazinyliminomethyl) rifamycin SV; 822.94 g / mol; CAS number: 13292-46-1

20 Toluene CAS number: 108-88-3

21 5-Sulfosalicylic acid hydrate CAS: 304851-84-1

22 ABTS; 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) Reagent part; CAS number: 30931-67-0

23 "Acrylamide / Bisacrylamide 29:11 for molecular biology - electrophoresis, 40% solution;

"

24 Acrylamide / Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 19: 1

1-Aminocyclopropane-1-carborylic acid; CAS number: 22059-21-8

26 1-Aminocyclopropane-1-carborylic acid; CAS number: 22059-21-8

27 Egg albumin, biochemistry powder, Ovalbumin; CAS number: 9006-59-1

28 L-ascorbic acid standard,> 99.0% crystalline CAS number 50-81-7

29 "6-BAP, BA, N6-Benzyladenine, C12H11N5

Synonyms: BA, N6-Benzyladenine, M 225; CAS number: 1214-39-7 "

Boric acid for molecular biology; CAS number: 10043-35-3

31 WellRED labeled oligonucleotides

32 Chloramphenicol; CAS Number: 56-75-7

33 Taq DNA Polymerase from Thermus aquaticus with 10xPCR reaction buffer without MgCl2; CAS number: 9012-90-2

34 Formaldehyde solution; contains 10-15% methanol as stabilizer, 37% wt in H2O; CAS number: 50-00-0

Formamide for molecular biology ≥99.5% (GC); CAS number: 75-12-7

36 Folin Reagent - Folin-Ciocalteu's phenol reagent 2N

37 Gallic acid purity 97.5%; CAS number: 149-91-7

38 SDS (sodium lauryl sulfate), for molecular biology, 100 grams, purity: nuclease-free (molecular biology grade), pH 6.5-8.5

39 5-Hydroxymethyl-2-furfuradehyde, 99%; CAS: 67-47-0

40 "Hexadecyltrimethylammonium bromide for molecular biology, synonyms: CTAB, Cetrimonium bromide, Cetyltrimethylammonium bromide, Palmityltrimethylammonium bromide CH3 (CH2) 15N (Br) (CH3) 3, M 364.45 g / mol; CAS: 57-09-0

"

41 "Indolylbutyric acid, synonyms: 4- (3-Indolyl) butanoic acid

4- (3-Indolyl) butyric acid, IBA, C12H13NO2, M 203.24 g / mol; CAS: 133-32-4 "

Isomaltose, 98% purity, HPLC chromatographic standard; CAS number: 499-40-1

43 Indolo3-carbinol analytical standard (Purity HPLC> 96%); CAS number: 700-06-1

44 L-malic acid standard,> 95.0%

Ninhydrin CAS number: 485-47-2

Due to the limited number of characters in the form, it is not possible to provide a full description. A full description of the subject of the contract is included in Annex 1.40 to the ToR "Offer form part 40"

"The kit is intended for fast and efficient isolation of high purity DNA from solid tissues (fresh, frozen, fixed in paraffin or embedded in paraffin), physiological fluids, hair, rodent tails, insects and cell lines.

RNase A and Proteinase K enzymes are provided in lyophilized form. Each tube contains the appropriate amount of enzyme to carry out 50 DNA isolations. Before first use, RNase A and Proteinase K lyophilisate should be dissolved in the appropriate kit buffers.

Elution is carried out using 100-200 ul Elution Buffer, which contains 0.5 mM EDTA. The isolation procedure does not require the activation of mini-columns.

PRODUCT SPECIFICATION

OUTPUT MATERIAL

• fresh or frozen solid tissue: 1-30 mg

• formalin-fixed tissue: 1-30 mg

• tissue in a paraffin block: 1-30 mg

• cell lines: 103-107 cells

• physiological fluids (urine, PMR, peritoneal fluid): 1-5 ml

• hair: 1–30 mg

• rodent tails: 1-30 mg

• insects: 1-30 mg

The product is intended for use only

For research purposes "

1 Proteinase K, a serine protease, used to digest proteins in nucleic acid preparations. It degrades proteins even in the presence of detergents. Proteinase K cleaves peptide bonds on the carboxyl side of aliphatic, aromatic or hydrophobic amino acids. The smallest peptide to be hydrolyzed by this enzyme is tetrapeptide.

2 DNA polymerase, concentration 5U / uL. The combination of DreamTaq DNA polymerase and 10X DreamTaq Green Buffer. DreamTaq DNA Polymerase is a reinforced Taq polymerase optimized for high throughput PCR applications. It provides greater sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. 10X DreamTaq Green Buffer contains a loading buffer and two dyes for direct loading of PCR products onto the gel after the PCR reaction. The colored buffer does not interfere with PCR efficiency and is compatible with further applications such as DNA sequencing, ligation and restriction digestion. The 10X DreamTaq buffer is optimized for performance in PCR and contains MgCl2 at a concentration of 20 mM. In the package 5 x 100 μL DreamTaq DNA Polymerase (5 U / μL) and 10 x 1.25 mL 10X DreamTaq Green Buffer (containing 20 mM MgCl2). Storage in -20 about C.

3 O'GeneRuler 100bp DNA Ladder Plus, ready-to-use. Ladder of size for O'GeneRuler electrophoretic separations, size range from 100 - 3000 base pairs, contains a total of 14 size standards, including 2 visibly thicker than others (sizes 500 and 1000 pz. Ready to use, supplied with 6x concentrated dye (Orange Loading Dye Solution) for loading samples.

4 Loading dye for electrophoresis 6 x concentrated "DNA Gel Loading Dye (6X)"

5 "T4 DNA ligase (5U / ul), catalyzes the formation of phosphodiester bonds between aligned 5'-phosphate and 3'-hydroxyl ends in DNA or RNA duplexes. The enzyme repairs single incisions in DNA, RNA duplexes or RNA / DNA hybrids by joining coherent or blunt DNA ends. However, it has no activity on single-stranded nucleic acids. T4 DNA ligase requires ATP as a cofactor. It allows ligation of sticky ends in 10 min at 100% activity in restriction enzyme buffers, PCR and RT

Ligase must be supplied with a reaction buffer containing ATP and 50% PEG for efficient blunt end ligation, strongly inhibited by NaCl or KCl if the concentration exceeds 200 mM, it is inactivated at 65 about C for 10 min. or in the 70's about C for 5 minutes. "

6 EcoRI restriction enzyme (10U / μL). Pack size of 5000 units. 10U / uL concentration. Enzyme recognizing the G ^ AATTC sequence most efficiently at 37 about C in EcoRI buffer. Convenient color-coded buffer system (B-blue buffer, G-green buffer, O-orange buffer, R-red buffer, Tango-yellow buffer). The kit contains a universal Tango double digestion buffer. BSA in reaction buffers. Sensitive to thermal inactivation (temp. 65 about C). Sensitive to methylation.

7 Hpa II restriction enzyme, 10U / uL concentration.

8 Restriction enzyme Tru1I (MseI), concentration 10U / µL, recognizes the T ^ TAA sequence, acting most efficiently at 65 about C in buffer R. Convenient color-coded buffer system (B-blue buffer, G-green buffer, O-orange buffer, R-red buffer, Tango-yellow buffer). The kit contains a universal Tango double digestion buffer. BSA in reaction buffers. Insensitive to thermal inactivation. Insensitive to methylation.

Due to the limited number of characters in the form, it is not possible to provide a full description. A full description of the subject of the contract is included in Annex 1.42 to the ToR "Offer form part 42"

D-isocitric acid enzymatic determination kit, IFU 54 acid determination kit

Syngen gel / PCR ME Mini Kit

Silver nitrate

Surface disinfectant based on Chloramine T (sodium salt), active chlorine content 25%.

INT-Mix 5 (10% HNO3w HCl) standard for ICP-MS

Sodium sulfate anhydrous extra pure fine powder Ph Eur, BP, USP., CAS: 7757-82-6

Phadeba Honey Diastase Test Tablets (Phadeba Honey Diastase Test Tablets for the determination of diastase number in honey)

1 Nutrient Agar, Medium to be used for breeding and determining the number of different microorganisms in water, sewage, stool samples and other Becton Dickinson quality materials.

2 "Bacto Agar. The purified product is characterized by a reduced amount of side substances, dyes and salts to a minimum. Used to study movement and to grow anaerobic bacteria

And microaerophilic. "

3 Pseudomonas Agar F, the medium also appears as Flo Agar, increases the production of fluorescein by Pseudomonas.

4 Difco Agar, Granulated with properties: humidity: ≤20%, ash ≤6.5%; Ca (ppm): ≤300-2500; Mg (ppm): ≤50-1000; melting point: 83-89 about C; gelation temperature: 32-39 about C.

5 Potato Dextrose Agar (PDA) - Potato dextrose agar; Dehydrated, PDA 39 g / 1 L Water: Potato starch 4g / L, Glucose 20g / L, Agar 15g / L; Autoclavable at 121oC for 15 minutes; pH 5.6 ± 0.2, Becton Dickinson quality.

6 LB Agar Miller (LBA) - Dehydrated; Tryptone 10g / L, yeast extract 5g / L, Sodium Chloride 10g / L, Agar 15g / L; Storage: 2-25o C; Autoclavable at 121oC for 15 minutes; pH 7.0 ± 0.2

1 Standard II seven (7) anionic Standard to IC - 7 anionic in water, with specified uncertainty

2 Ion-selective electrode fluids reference for Orion type electrodes

3 Ion selective electrode fluids reference for Orion type electrodes

4 Ion-selective electrode fluids reference for Orion type electrode with ammonium

1 Calibration solution for the ICP-MS apparatus containing the elements: Ba, Be, Bi, Ce, Co, In, Li, Ni, Pb, U at a concentration of 10 μm / ml each of these elements is contained in a 5% NHO3 aqueous solution (v / v). The value of the elements listed must be given with certain uncertainty. The calibration solution must have a reference to the international certified standards NIST, CRM, SRM

2 ICP internal standard 10 μg / ml in HNO3 Standard for ICP-MS

1 Plant Propagation LAB-AGAR

2 Yeast extract is an autolysate of yeast cells, enriching many solid and liquid bacteriological media for microbial culture. Yeast extract is a source of amino acids and peptides, water-soluble vitamins, in particular B vitamins, purines, pyrimidines, carbohydrates and mineral salts. According to USP recommendations, it is used in sterility tests. The product is in the form of microgranules, beige-yellow color.

3 Kit containing 95 reagents placed on a microtiter plate, designed to identify gram positive and gram negative bacteria. The set should be compatible with the GEN III MicroStation System identification and characterization system, catalog number 65361.

4 Kit containing 3 x 31 reagents placed on a microtiter plate, designed to assess the activity and biodiversity of microorganisms. The set should be compatible with the GEN III MicroStation System identification and characterization system, catalog number 65361.

5 Universal fluid for suspending bacterial cells in accordance with the bacterial identification protocol of the system for identification and characterization of microorganisms GEN III MicroStation System cat. No. 65361.

6 Fluid for suspending bacterial cells (gram positive bacteria, gram negative envelope-producing bacteria and having strong reducing properties) in accordance with the protocol for identification and characterization of microorganisms GEN III MicroStation System cat. No. 65361.

8 Agar medium for growing aerobic bacteria for identification with the Biolog system

Disinfectant, fungicidal and bactericidal liquid, designed for disinfecting laboratory devices, tools, rubber and plastic materials. It should not contain aldehydes. 15 minutes exposure time of 0.25% fluid solution should be bactericidal, 15 minutes exposure time of 2% fluid solution should be fungicidal.

1 Resin AG 1-X8 Resin acetate form Ion exchange resin in acetate form From the binding of organic acids

2 Real-time reagent kit containing: 2x concentrated, ready-to-use real-time PCR mix, fluorescent dye, hotstart Sso7d fusion polymerase, nucleotides, magnesium, SYBR Green I dye, enhancers, stabilizers and passive reference dyes (ROC and fluorescein). The set is compatible with the BioRad CFX thermal cycler and other real-time PCR thermal cyclers, cooperating with the ROX dye (e.g. Roche LightCycler LC480, Qiagen Rotor-Gene Q, Eppendorf Mastercyclers and Stratagene Mx real-time PCR.

BSA liquid / suspended in distilled water, bovine serum albumin