The subject of the order is the delivery of genetic reagents for the Institute "CZMP"
Package No. 1
1. Fetal serum from South American cattle, heat inactivated, sterile, tested for mycoplasma, endotoxins, 1 op. = 500 ml. - 9,000 ml
Package No. 10
1. Betaine 5M ultra-pure, 5x1.5mL packed in molecular biology. - 38 ml
Package No. 11
1. Reagent for staining nucleic acids without toxic ethidium bromide. Excellent sensitivity in visualization with UV or blue light excitation. When bound to nucleic acids, green fluorescence has a maximum fluorescence at 280 and 502 nm and a maximum emission at 530 nm. Supplied as a 10,000x concentrate in DMSO. The dye can be mixed with agarose gel. Packed on 400 street. - 12,000 μl
2. A dye used to prepare DNA markers and samples for placement in an agarose gel. It contains two different dyes - bromophenol blue and FF xylocyanide to visualize the tracking of DNA migration during electrophoresis. Contains EDTA which binds metal ions and inhibits nucleases. Concentrated 6x, packed 5 x 1 ml. - 25 ml
Package No. 12
1. Mass standard with a range of 100 - 1000 bp; Significant mass range and clear distribution of individual mass sizes in agarose gel, ready for use. Packed in 50 ug (100 applications). - 500 μg
2. Mass standard in the range of 50 - 1000 bp; Significant mass range and clear distribution of individual mass sizes in agarose gel, ready for use. Packed in 50 ug (100 applications). - 1,000 μg
3. Weight standard in the range of 10 - 300 bp; Significant mass range and clear distribution of individual mass sizes in agarose gel, ready for use. Packed in 50 ug (100 applications). - 250 μg
Package No. 13
1. dNTP mix of 10 mM each dATP, dCTP, dGTP, dTTP. Greater purity than 99% confirmed by HPLC, nuclease free, E. coli, highly stable. Stability for years at -20 degrees, you can repeatedly thaw and freeze. It remains in the form of triphosphates after 30 cycles of PCR (1 minute at 94st, 3 minutes at 72st). Packed in 1 ml. - 15 ml
Package No. 14
1. Ready-to-use green multiplex master mix, resistant to inhibitors, specific and effective for multiplexing up to 2.5 kb. It has two dyes that allow you to visualize gel products directly. Based on Phusion Hot Start polymerase. It can even be used for crude samples, such as blood or serum. Pack size of 100 reactions. - 2,000 reactions
Package No. 15
1. Ready-to-use cDNA synthesis kit, with short reaction time (10 minutes), high synthase capacity in the presence of inhibitors, with reduced RNAse H activity, containing random oligonucleotide and oligo (dT) 20 primers, with removal gDNA pollution. Pack size of 50 responses. - 1,000 reactions
Package No. 16
1. Kit for cDNA synthesis with primers of random oligonucleotide type. The size of 200 reactions. - 4,000 reactions
Package No. 17
1. Ready-to-use PCR reaction mastermix for RT-qPCR, optimized for gene expression testing and validated for miRNA expression testing, containing ROX as a passive dye, optimized for work with hydrolyzable probes (TaqMan). Packed in 5 ml. - 100 ml
2. RT-qPCR mastermix ready for use in the PCR reaction, optimized for gene expression testing, containing DNA Urydylotransferase, ROX as a passive dye and SYBR Green. Packed in 5 ml. - 100 ml
Package No. 18
1. Phytoheaemglutynin PHA HA 15 - stimulator of cell division - lyophilized phythemagglutinin in 5ml packs - 1 500 ml
Package No. 19
1. Trypsin powder, 1: 250, pack size of 100g. Mixture of proteases derived from pig's pancreas. Due to its digestive power, it is used to conduct cell cultures. Trypsin tested for endotoxin, mycoplasm, bacteria, viruses and fungi contamination. - 200 g
Package No. 2
1. 0.25% trypsin solution in PBS without calcium, magnesium and phenol red, sterile, 1 pack = 500 ml. - 10,000 ml
Package No. 20
1. Kit for isolating DNA from peripheral blood for the MagCore automaton - 9,600 reactions
2. Set for DNA isolation from cell cultures and amniotic fluid for the MagCore automaton - 768 reactions
3. Kit for isolation of total RNA from peripheral blood for the MagCore automaton - 192 reactions
4. Kit for total RNA isolation from culture cultures for a MagCore automaton - 192 reactions
5. Tissue DNA isolation kit for the MagCore automaton - 192 reactions
6. DNA isolation kit from forensic samples, such as a dried blood spot, swab samples for the MagCore automat - 192 reactions
7. Free-circulating DNA isolation kit for the MagCore machine with the required additional rack enabling a larger volume of the input sample (4 ml). - 192 reactions
8. Kit for purifying and concentrating DNA from enzymatic reactions based on the use of magnetic beads. Kit for purifying specific ranges of DNA fragments from PCR, endonuclease digestion, ligation, removal of adapters, etc. The kit allows selective binding of DNA fragments based on the volume of buffer added to the sample. The kit allows elution of ≥ 25 μl DNA when using 96-well plates and ≥ 10 μl DNA when using tubes. The eluate can be used for ligation, restriction digestion, next generation sequencing, and library preparation. The volume of the buffer included in the kit is not less than 10 ml. The kit allows you to carry out the entire procedure in 10 minutes. The yield of DNA obtained is not worse than ≤ 10 μg DNA Size range ≥ 100 bp. - 50 ml
9. Free circulating DNA (cfDNA) stabilizing reagent for up to 14 days, genomic DNA (gDNA) and free circulating tumor cells (CTCs) for up to 7 days, in the temperature range of 6-37 degrees Celsius, in ready glass tubes with a vacuum blood collection system. Capacity: 9.8 ml blood, 1.8 ml reagent - 800 pcs.
Package No. 21
1. Standard agarose - 100bp - 25kB. Packed in 500g. - 5,000 g
2. High resolution agarose - 20 - 800bp. Packed in 100g. - 600 g
3. Standard agarose reagent kit with ready-to-use 10xTBE buffer compatible for horizontal electrophoresis apparatus with electrodes for connection to a power supply, sledges for pouring gel 15x15 cm, two combs 1mm thick and the number of teeth 20 and pour 20x10 cm, 2 x comb with 20 teeth 1mm wide. The kit also includes 2 combs with 40 teeth 1 mm thick. - 1 op
Package No. 22
1. DEPC treated water, sterile, highly purified for use in PCR technology. The product in ready portions of 50 x 5ml, which prevents contamination. - 1000 ml
2. Ready-to-use buffer 10x TBE for horizontal electrophoresis, containing Tris, boric acid and EDTA, 1 liter each. - 20 L.
Package No. 23
1. QuantiFluor ONE - reagent for measuring the concentration of nucleic acids dedicated to the Promega Quantus spectrophotometer. 500 reactions. - 2,000 reactions
2. Master Mix PCR - ready to use in the PCR reaction mastermix containing HotStart polymerase, dNTP, MgCl2, buffer and 2 dyes for electrophoresis: blue (it travels like 3-5kb in 1% agarose gel) and yellow (it travels like <50 bp in 1% agarose gel), ready for direct application on a gel after PCR. Size of 1000 reactions. - 10,000 reactions
3. Heat-inactivated HhaI restriction enzyme, specific to MS-MLPA; concentration 10 U / μl, op. = 1000 U, compatible with MRC-Holland ME028 PWS / AS probe set - 4,000 U
Package No. 24
1. A kit for concentrating and purifying free circulating DNA, RNA, miRNA and viral nucleic acids from human plasma, serum, urine or other extracellular fluids
Fluids. Sample volume isolated with the kit: 1-5ml. Elution volume 20-150 μl. Isolation time ˂ 2h for 24 samples. The set is intended for use with a vacuum pump that provides abs vacuum. 8 mbar. The kit contains the original manufacturer's protocol.
The kit includes the following reagents: silica columns 50 pieces, 20 ml adapters 2 x 25 pieces, test tubes (2.0 ml) 50 pieces, elution tubes (1.5 ml) 50 pieces, tap adapters 50 pieces, ACL buffer 220 ml, ACB buffer ( concentrated) 300 ml, buffer ACW1 (concentrated) 19 ml, buffer ACW2 (concentrated) 13 ml, AVE buffer 5 x 2 ml, proteinase K 4 x 7 ml, carrier RNA 310 μg. Volume for 50 reactions. - 800 reactions
2. Kit for free-circulating DNA isolation, containing silica columns 50 pieces, 20 ml adapters 2 x 25 pieces, test tubes (2.0 ml) 50 pieces, elution tubes (1.5 ml) 50 pieces, tap adapters 50 pieces, ACL buffer 220 ml, buffer ACB (concentrated) 300 ml, ACW1 buffer (concentrated) 19 ml, buffer ACW1 (concentrated) 13 ml, AVE buffer 5 x 2 ml, proteinase K 4 x 7 ml, carrier RNA 310 μg and adapter for nucleic acid isolation : tripod for 24 insulation columns, tray made of polypropylene, which has a sterile filter, vacuum pressure indicator, vacuum regulator, vacuum pump location, hose connecting to the vacuum pump, tightly closed waste bottles, cables connecting individual elements of the system , pump - 1 pack
3. Multiplex PCR kit, without the need for optimization with high sensitivity and specificity, by using hot start. Easy to use. Ready-to-use kit in one master mix, containing HotStarTaq DNA polymerase and a unique buffer containing MP factor. It stabilizes binding of primers and increases the efficiency of sequence extension without the required optimization (optimized magnesium, dNTP co-concentration, buffer). In addition, the set includes a Q-solution that enhances the reaction of difficult fragments (e.g. rich in GC pairs). Polymerase concentration 5units / ul, 2-4 kb / min at 72∘C. 100 reactions. - 500 reactions
4. Kit for manual isolation of genomic DNA from solid tissues, swabs, mr fluid, blood, fluids
Body urinary sediment cells; The set is based on the use of DNA binding silica membranes. Columns with silica membranes catch DNA from
Yield 99%. DNA is desalted and concentrated by alcohol precipitation. After concentration and desalting, DNA has the highest degree of purification and can be suspended in any volume of water or a suitable buffer. DNA can be isolated from 25 mg of tissue, or up to 200 ul of fluid per 20
Minutes and suspend in 50-200 ul of proper buffer. Without the need for mechanical homogenization, tissues undergo enzymatic lysis. DNA purity after elution min 1.8. Kit for 50 isolations. The kit includes the following reagents: silica columns 50 pieces, 2 ml tubes 150, AL buffer 12 ml 2 x 33 ml, ATL buffer 14 ml, buffer AW1 (concentrated) 19 ml, buffer AW2 (concentrated) 13 ml, buffer AE 2 x 15 ml, K proteinase 1.25 ml. The size of 50 reactions. - 250 reactions
Package No. 25
1. Primers for the amplification and sequencing of selected genomic DNA fragments 0.02 μl / mol, max. 30NN
1 pack = up to 30 nucleotides (NN)
* for quotations, it should be assumed that 1 packet contains 30 NN. The orderer will order the amount of nuclotides in the package depending on the needs. The price will be calculated from the proportions. - 55,800 NN
Package No. 26
1. Expand Long Template PCR set for the amplification of long fragments from genomic DNA. The optimal combination of enzymes and specially developed dedicated three buffers allows amplification of products up to 20k in length. Contains a mixture of enzymes - thermostable Taq DNA polymerases. It allows three times more accurate amplification than using one Taq DNA polymerase. In a package of 5 U / ul enzyme mix, and 3 buffers concentrated 10x 17.5mM MgCL2, 27.5mM and 27.5 mmM with additional reagent. Size 150 U - 600 U
2. Expand Long Template PCR set for the amplification of long fragments from genomic DNA. The optimal combination of enzymes and specially developed dedicated two buffers allows amplification of products up to 20k in length. It contains a mixture of enzymes - thermostable Taq 175U DNA polymerases. It allows three times more accurate amplification than using one Taq DNA polymerase. The kit contains a buffer with 12.5 mM MgCl2 5x concentrated and a buffer without magnesium, slope 25 mM MgCl2, DMSO 100%, PCR dNTP mix. Size 175 U - 1,750 U
Package No. 27
1. Ready-to-use red mix for high-throughput PCR with REDTaq polymerase. Fast PCR performance. The mix contains Taq polymerase, 99% pure nucleotides, buffer, neutral red dye in a 2x optimized reaction concentrate. Can be loaded directly onto agarose gel without loading buffers. It is used for PCR multiplexing and reduces the in specificity of the reaction. 100 reactions. - 1,000 reactions
Package No. 28
1. Collagenase is an enzyme with high activity that digests collagen. It can operate at lower temperatures (4-10 degrees C), packaging size 100mg. Collagenase derived from Clostridium histolyticum is used in cell cultures in the preparation of cell suspensions. - 400 mg
Package No. 29
1. SALSA MLPA probemix - 25 reactions
1 pack = 25 reactions - 1 500 reactions
2. SALSA MLPA probemix - 50 reactions
1 pack = 50 reactions - 1 500 reactions
3. SALSA MLPA - 100 reactions
1 pack = 100 reactions - 4,000 reactions
Package No. 3
1. PBS solution without calcium and magnesium, colorless, sterile, 1 pack. = 500 ml. - 85000 ml
Package No. 30
1. 0.5 M EDTA buffer, pH 8.0 (ultrapure). Packed in 100 ml. - 400 ml
2. TE buffer 1x concentrated, pH = 8.0, nuclease free. Packed in 500 ml. - 3000 ml
3. Hot-StarTaq DNA polymerase
- polymerase with HotStar activity, concentration 5 U / μl
- active site blocked due to a unique chemical modification, the enzyme remains completely (100%) inactive at ambient temperature
- the kit includes a special buffer for performing PCR reactions using difficult matrices, rich in GC pairs, not containing DMSO
- activation time 15 minutes 95
- set contains 4 x 250 units HotStarTaq DNA Polymerase, 10x PCR Buffer, 5x Q-Solution, 25 mM MgCl2 - 12,000 U
4. Thermostable DNA polymerase ensuring amplification of short and medium templates in a set with 10x, 50 mM MgCl2 buffer. 500 reactions. - 7,500 reactions
5. Multiplex PCR Master Mix (2x) - ready to carry out PCR-multiplex Master Mix reaction, containing Perpetual Taq HotStart polymerase; packaging for 100 reactions of 50 μl - 800 reactions
Package No. 31
1.3% Agarose, dye-free, w / internal standards, BluePippin, 100-250 bp, 10 / pk, Marker Q3 - a complete set of gel cassettes and reagents compatible with the BluePippin device allowing the selection of DNA fragments in the range of 100-250 bp . Kit for 50 samples. For your BluePippin camera. - 600 samples
2.2% Agarose, dye-free, w / internal standards, BluePippin, 100 - 600 bp, 10 / pk, Marker V1 - a complete set of gel cassettes and reagents compatible with the BluePippin device, allowing the selection of DNA fragments in the range of 100-600 bp. Kit for 50 samples. For your BluePippin camera. - 500 samples
Package No. 32
1. The main set of oligonucleotides with the Mix Panel v4-2JK number (173222, IAA18658_1000), designed for the proprietary On-Demand gene panel for NGS sequencing; reagent volume of a set of 96 reactions; compatible with MiniSeq (Illumina) device - 192 reactions
2. A complementary set of oligonucleotides with the number SpikeIn_To_IAA18658-2 (173223, IAA18660_182, Amplicons 229) designed for an original panel of genes in the On-Demand system for NGS sequencing; reagent volume of the 3000 reaction set; compatible with MiniSeq (Illumina) device - 3000 reactions
3. A set of reagents for creating libraries in the Library PLUS system dedicated to the proprietary gene panel created in the On-Demand system for sequencing with the NGS technique; reagent volume of 96 reactions; compatible with MiniSeq (Illumina) device - 192 reactions
4. Set A - 96 dedicated idexes for the AmpliSeq reagent system for NGS sequencing; reagent volume of 96 reactions; compatible with MiniSeq (Illumina) device - 96 reactions
5. Set B - 96 dedicated idexes for the AmpliSeq reagent system for NGS sequencing; reagent volume of 96 reactions; compatible with MiniSeq (Illumina) device - 96 reactions
6. A set of reagents consisting of three elements: cartridge (in High Output format, 300 cycles), flow cell, hybridization buffer; compatible with MiniSeq (Illumina) device - 192 reactions
Package No. 4
1. Inhibitor of cell division with a concentration of 10μg / ml in PBS, colorless, sterile, 1 op. = 10 ml. - 120 ml
Package number 5
1. Blue fluorescence dye used, among others in FISH technique - ready solution (0.125 ng / ml) - DAPI II Counterstain in Antifade. - 8 ml
2. Non-ionic reagent for the preparation of washing solutions used in the FISH technique. - 20 ml
3. Flexible removable adhesive based on natural rubber and organic solvents enabling gluing and resealing of the microscope slide and cover slide. Suitable for use, among others in the FISH technique. - 500 g
4. Molecular probe used in the FISH technique to diagnose microdeletion and microduplication in the 22q11.2 region on chromosome 22. Complementary to the TUPLE1 gene. Control probe in the 22qter region (ARSA gene). - 40 tests
5. Molecular probe used in FISH technique for diagnostics of microdeletion and microduplication in the 15q11.2 region on chromosome 15. Complementary to the SNRPN gene. Control probe in the 15qter region. - 30 tests
Package number 6
1. Complete medium for culture of amniotic fluid cells and trophoblast cells in a volume of 500 ml. Ready to use, for fetal prenatal diagnosis, providing breeding time of 6-8 days. - 45,000 ml
2. Complete medium for culture of amniotic fluid cells and trophoblast cells in a volume of 500 ml. Enriched medium inhibits the growth of epithelial cells, allowing for more metaphases to be obtained in a slightly longer time than BIO-AMF-2. The end of breeding is possible after 10-12 days. BIO-AMF-3 medium is often used for parallel cultures together with BIO-AMF-2. - 30,000 ml
Package No. 7
1. RPMI 1640 medium with stabilized L-glutamine, 25mM Hepes, sterile product with phenyl red in 100 ml - 70,000 ml packs
Package No. 8
1. Anode buffer container for Genetic Analyzer 3500 Applied Biosystems - 80 pcs.
2. Cathode buffer container for Genetic Analyzer 3500 Applied Biosystems - 80 pcs.
3. GeneScan 600 LIZ Size Standard v2.0 - fluorescent dye used to analyze 20-600 bp fragments, for Genetic Analyzer 3500 Applied Biosystems - 2,400 reactions
4. Buffer for BigDye Terminator v.3.1 Cycle Sequencing Kit from Applied Biosystems or equivalent required in the Sanger sequencing procedure, concentrated 5x - 50 ml
5. BigDyeTerminator 3.1 Cycle Sequencing Kit - DNA sequencing kit for the 3500 Genetic Analyzer Applied Biosystems (according to this reagent the device is calibrated); packaging for 100 reactions - 10,000 reactions
6. Formamide Hi-Di (highly deionized), required for DNA precipitation procedure, for capillary electrophoresis, for 3500 Genetiv Analyzer Applied Biosystems sequencer. Packed in bottles of 25 ml. - 375 ml
7. Glycogen - DNA precipitation reagent, highly purified (Molecular Biology Grade) polysaccharide isolated from oysters. It is an inert carrier that increases the extraction of nucleic acids from alcohol precipitation. Packed in 2x0.25 ml. - 10 ml
8. 3M sodium acetate, pH 5.2 - DNA precipitation reagent, free from Dnase and Rassa, filtered through a 0.22 m membrane, sterile. Packed with 5 x 1 mL. - 75 ml
9. ExoSAP - Reagent for enzymatic purification of products after PCR before sequencing by Sanger; providing one-step purification without having to combine exonuclease I and shrimp alkaline phosphatase; no more than 1μL per 2.5μL PCR product; 2 stage incubation: 37
10. POP-7 Polymer for Genetic Analyzer 3500 sequencer, size of 384 reactions - 3 840 reactions
11. POP-7 Polymer for Genetic Analyzer 3500 sequencer, size 960 reactions - 7 680 reactions
12. Genetic Analyzer 3500 Applied Biosystems sequencing calibration kit The BigDye Terminator v3.1 sequencing standard kit - 4 pcs.
Package No. 9
1. Hind III - a restriction enzyme with a concentration of 10 U / μ., Enzyme cleavage sites: 5´-A ↓ AGCTT-3´ 3´TTCGA ↑ A-5´, op. = 10,000 Units - 50,000 U
2. Buffer dedicated to the enzyme HindIII, Buffer R (10x). Packed in 5 x 1 ml - 10 ml