cryo workflow

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The advertised cryogenic workflow for preparing biological samples will include the following components: a high-pressure freezer (HPF) followed by freeze substitution at low temperature and a cryo chamber for ultra-thin sections.

In order to be able to monitor the biological activity of live cells microscopically at defined interaction and development points, the cells must be vitrified or cryofinized. Cell processes are suddenly stopped and preserved without destroying the native tissue structure by ice crystal formation. Freeze-substitution also makes vitrified cell and tissue samples accessible to the non-cryogenic analysis infrastructure. Gentle low-temperature embedding is to be used, which must not change the sample structure in order to transfer the samples to room temperature conditions. In addition, ultra-thin sections of vitrified cell and tissue samples are to be produced in a cryo chamber using the Tokuyashu or ceMovis process. The fine structure of the samples is made accessible to high-resolution microscopy and spectroscopy. The uniform workflow must guarantee the transfer of the highly sensitive samples under continuous cryogenic conditions.