The subject of this public procurement is the purchase and delivery of pharmaceutical and laboratory equipment in four lots for the needs of research group 3.2.1 "Group on Angiogenesis and Vascular Biology" and research group 3.1.2 "BAHLMAA: Biologically Active Heterocyclic Ligands and Metal Complexes with Antioxidant Activity" in the implementation of project BG-RRP-2.004-0004-C01: "Strategic Research and Innovation Program for the Development of the Medical University – Sofia", funded by NPVU. The estimated value and minimum technical requirements are detailed in the Technical Specification attached to the documentation.
The delivery includes the following:
- preparation of details and necessary elements for installation at the delivery location specified in this Contract;
- installation, assembly, trial testing (including calibration), and commissioning of the supplied equipment;
- training of the CLIENT's personnel for operating the supplied equipment;
- warranty maintenance of the supplied equipment and delivery of necessary parts and materials within the warranty period, starting from the date of signing the protocol for commissioning. The warranty maintenance of the supplied equipment includes, repair, and delivery of necessary parts and materials within the warranty period, starting from the date of signing the protocol for commissioning.
The public procurement includes four distinct lots:
Lot 1: "Next Generation Protein Sequencing System":
Lot 2: "Mass Spectrometer for Thin Layer Chromatography":
Multi-core processor, 32 GB RAM, 1 TB disk, 24‘ monitor, laser printer, operating system Windows 11.
The system must be fully equipped for routine operation.
Lot 3: "Raman Microscope":
Lot 4: "Raman Spectrometer":
Place of execution of the order:
- for lot 1 for research group 3.2.1, address: Sofia, Medical University - Sofia, Department of Medical Genetics,  St. George Sofia St. No. 1;
- for lots 2, 3, and 4 for research group 3.2.2, address: Sofia, Medical University - Sofia, Faculty of Pharmacy, Dunav St. No. 2.
Everywhere where the Technical Specification and/or other document in the procedure contains a specific model, source or specific process that characterizes the products offered by a specific potential contractor, as well as a trademark, patent, type or specific origin or manufacture that would benefit or eliminate certain persons or some products, it should be read "or equivalent/equivalents".
In the case of providing an equivalent, the participant must prove by appropriate means that the offered solutions meet the requirements of the technical specifications equivalently.

LOT-0001
Next Generation Protein Sequencing System.
Purchase and delivery of a Next Generation Protein Sequencing System for research group 3.2.1 "Group on Angiogenesis and Vascular Biology" in the implementation of project BG-RRP-2.004-0004-C01: "Strategic Research and Innovation Program for the Development of the Medical University – Sofia", funded by NPVU. The estimated cost and minimum technical requirements are detailed in the Technical Specification attached to the documentation.
A next-generation system for comprehensive sequencing, including:
preparation, sequencing, and analysis of proteins to the single molecular level, single amino acid sequences.
The technology should include color-coded N-terminal amino acid recognizers and semiconductor chips to detect the binding characteristics and order of binding of N-terminal amino acids, leading to unique kinetic signatures that can be used to differentiate and identify residual amino acids and post-translational modifications.
Integrated directly into the system analytical software, allowing for:
Creating execution plans and workflows for analysis.
Organizing individual experiments into projects.
Tracking the status of instruments remotely.
Automating data analysis for performances.
Visualizing results of protein sequencing.
Storing, retrieving, and sharing results with authorized users in a secured cloud environment.
Compatibility with other software for more complex and detailed data analysis.
Access to cloud-based software for additional data analysis of gene expression.
The delivery includes the following:
- preparation of details and necessary elements for installation at the delivery location specified in this Contract;
- installation, assembly, trial testing (including calibration), and commissioning of the supplied equipment;
- training of the CLIENT's personnel for operating the supplied equipment;
- warranty maintenance of the supplied equipment and delivery of necessary parts and materials within the warranty period, starting from the date of signing the protocol for commissioning.
Address of place of performance: Sofia, Medical University - Sofia, Department of Medical Genetics,  St. George Sofia St. No. 1.

LOT-0002
Purchase of a mass spectrometer for thin layer chromatography.
Purchase and delivery of a mass spectrometer for thin layer chromatography for research group 3.2.2 "BAHLMAA: Biologically Active Heterocyclic Ligands and Metal Complexes with Antioxidant Activity" in the implementation of project BG-RRP-2.004-0004-C01: "Strategic Research and Innovation Program for the Development of the Medical University – Sofia", funded by NPVU. The estimated cost and minimum technical requirements are detailed in the Technical Specification attached to the documentation.
Desktop, specialized system for obtaining mass spectra from plates for thin layer chromatography.
Extraction and dissolution of the contents of the spot in liquid phase and subsequent mass analysis of the liquid sample.
Time for a single measurement, including placing the plate for thin layer chromatography ≤ 2 minutes.
To ensure compatibility of all components and modules, they must be from the same manufacturer.
Mass range 10–2000 m/z or more.
Switching between positive and negative operating mode during a single measurement ≤ 50 milliseconds.
Ion source: ionization modes must include both ASAP/APCI and at least ESI for effective ionization of both nonpolar and polar compounds (in positive and negative mode).
Switching time between different modes and readiness of the new source for operation ≤ 5 minutes.
Replacement of ion sources without the need for de-vacuuming.
The sources and inlet capillaries must be removable for cleaning without stopping the system's vacuum.
Availability of a probe for analysis of liquid and solid samples.
Software for complete control of the system.
Computer configuration of the mass spectrometer with the following minimum requirements: Multi-core processor, 32 GB RAM, 1 TB disk, 24‘ monitor, laser printer, operating system Windows 11.
The system must be fully equipped for routine operation.
The delivery includes the following:
- preparation of details and necessary elements for installation at the delivery location specified in this Contract;
- installation, assembly, trial testing (including calibration), and commissioning of the supplied equipment;
- training of the CLIENT's personnel for operating the supplied equipment;
- warranty maintenance of the supplied equipment and delivery of necessary parts and materials within the warranty period, starting from the date of signing the protocol for commissioning.
Address of place of performance: Sofia, Medical University - Sofia, Faculty of Pharmacy, Dunav St. No. 2.

LOT-0003
Raman Microscope.
Purchase and delivery of a Raman microscope for research group 3.2.2 "BAHLMAA: Biologically Active Heterocyclic Ligands and Metal Complexes with Antioxidant Activity" in the implementation of project BG-RRP-2.004-0004-C01: "Strategic Research and Innovation Program for the Development of the Medical University – Sofia", funded by NPVU. The estimated cost and minimum technical requirements are detailed in the Technical Specification attached to the documentation.
Raman microscope for analysis and imaging.
The system must operate with at least 4 different lasers, each with a specific wavelength and diffraction grating.
The system must have minimum Class 1 safety.
The system must have at least the following features:
- autofocus;
- software-controlled calibration;
- automatic correction of fluorescence.
Confocal resolution in depth: ≤ 2 µm.
Aperture width – minimum 4 (four) software-selectable values.
Automated laser polarization – at least horizontal, vertical, and depolarization.
Excitation lasers: wavelength 532 nm with maximum power on the sample ≥ 10 mW.
The system must control the power of the laser on the sample, by setting a value with a step ≤ 0.1 mW. Control must be performed through an integrated power meter.
Possibility to add lasers with wavelengths of at least 455 nm, 633 nm, and 785 nm.
Availability of a camera for observing the sample.
Software for controlling the spectrometer, processing, and storing the obtained data.
Software for quantitative analysis.
The software must have a feature for tracking laser usage hours and monitoring operational lifespan.
The delivery must include glasses for safe operation with the Raman microscope.
The Raman microscope must be equipped with at least 50x and 50x extended magnification objectives.
The system must include a library with a minimum of 90 spectra of polymers.
Computer configuration of the Raman microscope with the following minimum characteristics: multi-core processor, 32 GB RAM, 1TB HDD, 28‘ monitor, Laser color printer, Windows 11 Pro or equivalent.
The system must be fully equipped for routine operation.
The delivery includes the following:
- preparation of details and necessary elements for installation at the delivery location specified in this Contract;
- installation, assembly, trial testing (including calibration), and commissioning of the supplied equipment;
- training of the CLIENT's personnel for operating the supplied equipment;
- warranty maintenance of the supplied equipment and delivery of necessary parts and materials within the warranty period, starting from the date of signing the protocol for commissioning.
Address of place of performance: Sofia, Medical University - Sofia, Faculty of Pharmacy, Dunav St. No. 2.

LOT-0004
Raman Spectrometer.
Purchase and delivery of a Raman spectrometer for research group 3.2.2 "BAHLMAA: Biologically Active Heterocyclic Ligands and Metal Complexes with Antioxidant Activity" in the implementation of project BG-RRP-2.004-0004-C01: "Strategic Research and Innovation Program for the Development of the Medical University – Sofia", funded by NPVU. The estimated cost and minimum technical requirements are detailed in the Technical Specification attached to the documentation.
Laser with a wavelength of 532 nm.
The laser must be equipped with the respective grating and filter.
Laser safety: class 1.
Spectral range: minimum 3500 – 50 cm-1, for spectral resolution ≤ 5 cm-1.
Spectral dispersion 2 cm-1 / pixel.
Maximum output power of the laser ≥ 100 mW.
Maximum laser power on the sample ≥ 40 mW.
Size of the laser spot on the sample ≤ 10 µm.
Area of examination of the sample from the minimum size of the spot to a minimum of 5x5 mm.
Automatic calibration of the spectrograph at regular intervals without requiring user intervention.
Possibility to add additional lasers with wavelengths of 455, 633, and 785nm without requiring upgrades to the existing hardware configuration of the spectrometer.
Apertures – minimum 4, software-selectable.
Automatic calibration of the spectrograph at a specific time interval.
The Raman spectrometer must be equipped with at least:
Safety glasses.
A universal holder platform that allows the placement of bottles with liquids and powders.
An accessory for taking spectra in reflection mode at 1800.
A holder for powder samples.
Software for qualitative analysis.
Software for quantitative analysis.
Dimensions of the spectrometer: no more than 95x56x45 (Width x Depth x Height).
Maximum weight of the spectrometer: up to 53 kg.
Computer configuration of the Raman spectrometer with the following minimum requirements: multi-core processor, 16 GB RAM, 1 TB HDD, 3 USB ports, operating system Windows 11 Pro or equivalent, 24' LCD monitor, laser printer.
The delivery includes the following:
- preparation of details and necessary elements for installation at the delivery location specified in this Contract;
- installation, assembly, trial testing (including calibration), and commissioning of the supplied equipment;
- training of the CLIENT's personnel for operating the supplied equipment;
- warranty maintenance of the supplied equipment and delivery of necessary parts and materials within the warranty period, starting from the date of signing the protocol for commissioning.
Address of place of performance: Sofia, Medical University - Sofia, Faculty of Pharmacy, Dunav St. No. 2.