Procurement of reagents for the needs of the Neurogenetics Unit for a period of twenty-four (24) months.

LOT-0001
Section 23 - NlaIII.
Section 23 - NlaIII Recombinant restriction endonuclease NlaIII, concentration 10,000 units/ml. To cut effectively in short digestions lasting 5-15 minutes (Time saver) but also in long digestions (overnight) without reduction of its activity. It must have the ability to be thermally inactivated by incubation at 65°C for 20 min. It should be provided with 10x special reaction solution rCutSmart in which it acts with 100% efficiency and additionally Gel Loading Dye, Purple (6x). The reaction solution should contain 100 µg/ml recombinant Albumin Molecular Biology Grade, instead of BSA and can be used with 100% efficiency in all high-fidelity restriction endonucleases. Packaging: 500 units, quantity 1500 (packaging of 3).

LOT-0002
Section 24 - NsiI-HF.
Section 24 - NsiI-HF Recombinant restriction endonuclease NsiI-HF, concentration 20,000 units/ml. It must be high fidelity for minimized star activity, to cut effectively in short digestions lasting 5-15 minutes (Time saver) but also in long digestions (overnight) without reduction of its activity. It must have the ability to be thermally inactivated by incubation at 80°C for 20 min. It should be provided with 10x special reaction solution rCutSmart in which it acts with 100% efficiency and additionally Gel Loading Dye, Purple (6x). The reaction solution should contain 100 µg/ml recombinant Albumin Molecular Biology Grade, instead of BSA and can be used with 100% efficiency in all high-fidelity restriction endonucleases. Packaging: 1,000 units, quantity 3000 (packaging of 3)

LOT-0003
Section 25 - Taq DNA.
Section 25 - Taq DNA recombinant polymerase, for amplification of DNA fragments up to 5 Kb. It should have a concentration of 5u/μl and be accompanied by an appropriate 10X buffer solution and a separate Mg solution with a concentration of 25 - 50 mM. Packaging of 500 units, quantity 10,000 (packaging of 20).

LOT-0004
Section 26 - Synthesis of primers 20-25 bases.
Section 26 - Synthesis of primers 20-25 bases, in a quantity of 40 nmol, desalted. The synthesis must be based on phosphoramidite chemistry with an efficiency greater than 99.5%. The synthesis company must be certified under ISO 9001:2015 and ISO 13485:2016. The delivery time should not exceed 3-4 working days. The primers are base-specific, quantity: 2,000 bases.

LOT-0005
Section 27 - Reagent for DNA sequencing analysis.
Section 27 - Reagent for DNA sequencing analysis. It should be based on the chemistry of labeled dideoxynucleotides, four dyes (BigDye Terminators). It should include control plasmid pGEM and M13 primer, packaging 100 rxns, KIT 100 REACTIONS, Quantity 4.

LOT-0006
Section 28 - Polyacrylamide polymer.
Section 28 - Polyacrylamide polymer in closed packaging for automated filling of capillaries suitable for capillary electrophoresis technology, and applications for DNA fragment length sequencing-determination, VIAL 960 REACTIONS, quantity 6.

LOT-0007
Section 29 - Size marker up to 1000bp containing DNA fragments ranging from 47-946 bp.
Section 29 - Size marker up to 1000bp containing DNA fragments ranging from 47-946 bp, labeled with fluorescent dyes for use in electrophoresis in a genetic analyzer, packaging 400 samples, VIAL 400 REACTIONS, quantity 2.

LOT-0008
Section 30 - Size marker up to 1000bp containing DNA fragments ranging from 35-500 bp.
Section 30 - Size marker up to 1000bp containing DNA fragments ranging from 35-500 bp, labeled with fluorescent dyes for use in electrophoresis in a genetic analyzer, packaging 800 samples, VIAL 800 REACTIONS, quantity 10.

LOT-0009
Section 31 - High-quality deionized formamide solution.
Section 31 - High-quality deionized formamide solution for the denaturation and preparation of samples. It should be compatible with DNA electrophoresis injection technology in capillary electrophoresis. It should be available in packaging of 25 ml, quantity 100 (packaging of 4).

LOT-0010
Section 32 - Kit containing the reagents and materials required for performing the following steps in the next-generation sequencing workflow..
Section 32 - Kit containing the reagents and materials required for performing the following steps in the next-generation sequencing workflow • preparation of enriched, template-positive Ion Sphere™ Particles and loading onto an Ion 540™ Chip using the Ion Chef system. • Sequencing the loaded Chips on an Ion S5™ Sequencer, Ion S5™ XL Sequencer or Ion GeneStudio™ S5 Series Sequencer. Each kit should contain all the materials required for loading and sequencing 8 chips and be compatible for use with libraries up to 200 bases. Kit of 8 reactions / quantity: 6 Kits.

LOT-0011
Section 33 - Kit 16 uniquely labeled oligonucleotides (Barcodes) suitable for the creation of DNA libraries.
Section 33 - Kit 16 uniquely labeled oligonucleotides (Barcodes) suitable for the creation of DNA libraries. It should allow the simultaneous mix of DNA libraries from 16 different samples before the clonal amplification of the libraries into beads with PCR in emulsion for performing simultaneous multiplex sequencing of the libraries using semiconductor technology. Kit of 160 reactions. KIT 160 REACTIONS, quantity 2.

LOT-0012
Section 34 - Ion 540 Chip Kit.
Section 34 - Ion 540 Chip Kit Chip suitable for use with the Ion S5 series systems, 60-80M reads/chip Packaging 8 chips/ quantity: 1 packaging.

LOT-0013
Section 35 - Reagent kit for measuring dsDNA concentration in Qubit fluorometer.
Section 35 - Reagent kit for measuring dsDNA concentration in Qubit fluorometer. Quantification range 0.2-100 ng. Packaging 100 reactions, quantity 2.

LOT-0014
Section 36 - Ready-to-use magnetic beads.
Section 36 - Ready-to-use magnetic beads that provide optimized size selection and PCR cleanup, removing small fragments such as dNTPs, salts, primers, and primer dimers. Suitable for NGS Cleanup, PCR Cleanup, PCR Purification, DNA Purification and Sequencing applications. The cleanup protocol must be optimized for DNA fragments larger than 90 bp, with a recovery rate of >90%. They should be stored at room temperature and available in 50ml packaging. / quantity: 100 ml (2 packages)

LOT-0015
Section 37 - Kit for cleaning Dye terminators from sequencing reaction mixtures with spin columns.
Section 37 - Kit for cleaning Dye terminators from sequencing reaction mixtures with spin columns: Achieve effective removal of dye terminators (e.g. BigDye™ terminators) without precipitation with ethanol. The removal should be done with centrifuge columns (spin columns). It should include 2 ml collection tubes. Each column should be suitable for cleaning an amount of at least 20 μl from the sequencing reaction mixture. The cleaning time should be up to 5 minutes for each sample. It should be available in packaging of 250 reactions/cleanings, kit 250 reactions/cleanings, quantity 3.

LOT-0016
Section 38 - Kit for DNA isolation from PCR reaction or agarose gel /250 reactions.
Section 38 - Kit for DNA isolation from PCR reaction or agarose gel /250 reactions: PCR product cleaning and gel extraction should be achieved using the same kit with the same buffer. The procedure should be completed in less than 15 minutes. It should provide high DNA recovery even from very small pieces (>50bp). Complete removal of primers should be achieved. Small elution volumes from 15 μl up to 30 μl should be possible. It should use Silica Membrane technology with spin columns. It should provide ready-to-use DNA, suitable for cloning, sequencing, PCR, transformation, restriction analysis. ssDNA and SDS-containing samples isolation should be possible. It should include a DNA binding solution with pH indicator for optimal kit performance. It should include columns, and all necessary buffers. It should also be suitable for use with a vacuum manifold. It should be available in packaging of 250 reactions/cleanings, kit 250 reactions/cleanings, quantity 2.

LOT-0017
Section 39 - Certified Kit for the detection of GAA repeats in the first intron of the frataxin (FXN) gene.
Section 39 - Certified Kit for the detection of GAA repeats in the first intron of the frataxin (FXN) gene, which are the main cause of Friedreich's Ataxia (FA). It can show both the expansion of repeats in affected individuals and in carriers of the disease. It detects the size of the expansion, with capillary electrophoresis of fewer than 220 repeats and with the ability to detect more than 220 repeats in gel agarose, thus accurately determining the severity of the disease. The kit contains all necessary reagents for both capillary electrophoresis and gel agarose. Specifications: • has CE-IVD marking • is based on the PCR method using fluorescence for the detection of GAA repeats in the first intron of the frataxin (FXN) gene • detects both the expansion of repeats in affected individuals and in carriers of the disease • detects the size of the expansion, determining the severity of the disease • has an integrated panel and bins for easy interpretation of the repeats and determination of alleles. • is compatible with Applied Biosystems® 3130/3130xl, 3500/3500xl or Spectrum Compact CE System Promega genetic analyzer systems. • the DNA sample can be isolated from whole blood or amniotic fluid or chorionic villi or be cell-free DNA in amniotic fluid. • full analysis of 5-color (5-dye fragment analysis) with capillary electrophoresis. • the kit contains all reagents required for the analysis of 50 samples, such as 1200 Size Standard, Matrix Standard as well as PCR Mix, Primer Mixes, Extend Long DNA Polymerase, Enhancer Buffer, Control DNA, Carrier Control. Kit for 50 reactions, quantity 3.

LOT-0019
Section 40 - Ready mix for PCR.
Section 40 - Ready mix for PCR. It should be suitable for amplifying complex GC-rich samples, which cannot be amplified with conventional PCR techniques. It should be suitable for amplifying fragments up to 6 Kb, without errors. It should contain Advantage 2 Polymerase Mix, GC-Melt Reagent, and buffer with DMSO. It should be available in packaging for 100 reactions, Kit of 100 reactions, quantity 6.

LOT-0020
Section 41 - Pre-prepared high-fidelity PCR mix.
Section 41 - Pre-prepared high-fidelity PCR mix. It should have a concentration of at least 2X. It should include in the same mix Hi Fidelity polymerase hot start (0.5 units per 25 μl reaction), MgCl2 (25mM in 1X) and dNTPs (0.3 mM in 1X) so that for the reaction it is enough to add template DNA and primers. It should be suitable for amplifying fragments up to 15 kb when using genomic DNA as template. It should have an error rate of 3.6 x 10^6 or better. Activation of the hot start polymerase with exposure to high temperature should be completed in 20 sec or less. In packaging of 100 reactions of 25μl, Kit of 100 reactions, quantity 3.

LOT-0021
Section 42 - Polymerase.
Section 42 - Polymerase suitable for amplifying small, medium and large DNA fragments with increased fidelity. It should be an antibody-based HotStart DNA Polymerase for minimizing by-products. It should have 2x - 4x greater fidelity than Taq polymerase. It should have a concentration of 2.5 U/μl. It should also be suitable for the amplification of small initial quantities of DNA. The packaging should include: 5x LongRange Buffer 25 mM MgCl2 solution dNTP Mix (10 mM each). The Kit should be available in packaging of 100 units, Kit of 100 units, quantity 4.

LOT-0022
Section 43 - Ready-made polymerase and dNTPs mix for PCR (2X).
Section 43 - Ready-made polymerase and dNTPs mix for PCR (2X), with two inner tracking dyes. It should be a Hot Start polymerase mix with modified archaeal (Type B) DNA polymerase. The polymerase should be suitable for amplification of up to 20 kb with excellent sensitivity and increased fidelity. It should have 3x - 4x greater fidelity than Taq polymerase. It should also be suitable for the amplification of small initial quantities of DNA. It should be available in packaging of 100 reactions (volume of 25 μl), Kit of 100 reactions, quantity 5.

LOT-0023
Section 44 - Kit for total DNA isolation.
Section 44 - Kit for total DNA isolation (including genomic, mitochondrial, and viral DNA) from up to 400μl of starting sample blood, serum, plasma and buffy coat. It should be suitable for use in the automatic device MagCore. It should be suitable for DNA isolation from blood processed with sodium citrate, EDTA, lithium heparin, sodium fluoride. It should use magnetic beads technology. All necessary buffers, magnetic beads and Proteinase K should be included in pre-filled cartridges. It should provide high-purity DNA: A260/280 > 1.85. The elution volume should be from 60 to 200 μl. The process should be completed in less than 60 minutes. It should be available in packaging of 96 reactions/isolation, kit for 96 reactions/isolations, quantity 24.

LOT-0024
Part 45 - Certified Kit for the detection and amplification of CGG repeats in the 5' untranslated region (5' UTR) - FMR1.
Part 45 - Certified Kit for the detection and amplification of CGG repeats in the 5' untranslated region (5' UTR) of the FMR1 gene (Fragile X mental retardation 1). This kit contributes to the diagnosis of Fragile X syndrome and other Fragile X-related disorders, such as Fragile X-associated primary ovarian insufficiency (FXPOI) and Fragile X-associated tremor/ataxia syndrome (FXTAS), as well as to the identification of carriers of Fragile X syndrome. The kit uses three primers, including a pair of site-specific primers (forward and reverse) and a primer with multiple binding sites within the CGG repeats. The site-specific primers amplify full-length gene products, while the CGG primer generates the characteristic peaks on the electropherogram from alleles with CGG expansions. The size of the full-length PCR products is converted into the number of CGG repeats using a specific mathematical formula provided, and the categorization of alleles can be accurately determined based on the number of CGG repeats.
Specifications:
• CE-IVD marking
• PCR method using fluorescence for the detection of triplets, followed by capillary electrophoresis using a genetic analyzer to measure fragment sizes.
• It has an integrated panel and bins for result analysis that facilitate the interpretation of repeats and the determination of alleles.
• Compatible with Applied Biosystems® 3130/3130xl, 3500/3500xl, or 3730, 3730xl DNA genetic analyzer systems.
• Sample must be DNA isolated from whole blood.
• The kit contains all reagents required for the analysis of 100 samples, such as ROX 1000 Size Ladder, FMR1 Primer Mix, GC-Rich Amp Buffer, Polymerase Mix.
Kit for 100 reactions, quantity.

LOT-0025
Part 46 - Certified Kit for the detection of CTG repeats - DMPK - (MD1).
Part 46 - Certified Kit for the detection of CTG repeats in the non-coding region of the DMPK gene associated with Myotonic Dystrophy Type 1 (MD1). The size of the expansion is determined, thus indicating the severity of the disease. Fragment analysis is performed using capillary electrophoresis, and the number of repeats is calculated with a specific formula.
Specifications:
• CE-IVD marking.
• Full analysis 5-color (5-dye fragment analysis) with capillary electrophoresis.
• It has an integrated panel and bins for result analysis that facilitate the interpretation of repeats and the determination of alleles for identifying the severity of the disease based on the number of repeats.
• Compatible with Applied Biosystems® 3130/3130xl, 3500/3500xl genetic analyzer systems.
• Sample DNA that has been isolated from whole blood or blood from filter paper (DBC™) or diluted blood with the specific lysis buffer contained in the kit.
• The kit contains all reagents required for the analysis of 50 samples, such as 600 Size Standard, Matrix Standard, PCR Mix, polymerase, Control DNA & Primer mix.
Kit for 50 reactions, quantity 2.

LOT-0026
Part 47 - Ready-to-use certified Kit, suitable for In Vitro.
Part 47 - Ready-to-use certified Kit, suitable for In Vitro diagnostic use for the detection of CAG repeat triplets in types 1, 2, 3, 6, and 7, as well as CTA/CTG repeats in type SCA 8 of spinocerebellar ataxia. The Kit is based on PCR – fluorescent fragment Analysis technology and is compatible with ABI genetic analyzers: 3130, 3730 & 3500.
Kit for 16 reactions, quantity 8.