Acquisition of a spatial transcriptomics platform for gene expression analysis on tissue.
The instrumentation must meet the following characteristics:
- In situ analysis at single molecule/single cell
- Detection of RNA molecules (with dedicated panels) and proteins directly in the tissue, without dissociation.
- Decoding through cycles of fluorescent imaging and barcoding, with subcellular resolution (the distribution of the transcript within the cell can be seen).
- "Targeted" approach
- Use of pre-designed or custom probe panels targeting hundreds/thousands of genes.
- Presence of "disease/organ-focused" panels (e.g. oncology, immune, neuro, etc.) with a set of highly informative core genes expandable through customization
- Compatibility with FFPE and frozen tissues (human and murine)
- Optical resolution of at least 200nm
- Image format compatible with the software of the instrument manufacturer or third-party software with a volume not exceeding 200G
The purchased instrumentation must also allow:
- Analysis of fixed and paraffin-embedded tissues (FFPE) and fresh frozen tissues in isopentane preserved in OCT (Fresh Frozen) or dozens of Tissue Micro Array (TMA);
- Processes up to two slides in parallel;
- Scanning an area of 12 x 24 mm per slide;
- Image acquisition through a system utilizing 4 light sources;
- Cellular segmentation through 4 acquisition modes: Membrane Proteins, Cytoplasmic Proteins, Ribosomal RNA, Nuclei;
- Optical resolution: 200 nm, extendable to 30 nm thanks to image deconvolution software;
- The use of tissue-specific panels completely validated by the supplier for the detection of RNA and proteins;
- Non-destructive tissue workflow and fully compatible with downstream tissue analysis methodologies most commonly used in research laboratories;
- Use of circularizing probes to detect mRNA targets of interest;
- Use of probes amplification steps to increase the signal/noise ratio and make the analysis more sensitive;
- Tissue-specific pre-validated panels by the supplier for RNA detection allowing the analysis of up to 380 genes in parallel;
- Multi-tissue panels pre-validated by the supplier for RNA detection allowing the analysis of more than 5000 genes in parallel;
- Customization of pre-designed panels by adding up to 100 genes or creating completely custom panels up to 480 genes;
- Analyze up to 27 proteins using a pre-designed panel;
- Use of software for analysis and visualization of results (open-source and provided free of charge);
- Reduced data sizes not exceeding 200 GB;
- Usability of acquired data without the need to be transferred to an external Cloud, remaining fully compliant with privacy laws (GDPR) in the case of analysis of human samples

LOT-0001
NOTICE OF PRELIMINARY MARKET CONSULTATION PREPARATORY TO THE INITIATION OF A NEGOTIATED PROCEDURE WITHOUT THE PUBLICATION OF A TENDER PURSUANT TO ART. 76 PAR. 2 LETTER B), POINT 2 OF D.LGS. 36/2023 FOR THE SUPPLY OF A SPATIAL TRANSCRIPTOMIC PLATFORM FOR GENE EXPRESSION ANALYSIS ON TISSUE.
CUP B53C25003910001 - CUI F80209930587202500032.
Acquisition of a spatial transcriptomics platform for gene expression analysis on tissue.
The instrumentation must meet the following characteristics:
- In situ analysis at single molecule/single cell
- Detection of RNA molecules (with dedicated panels) and proteins directly in the tissue, without dissociation.
- Decoding through cycles of fluorescent imaging and barcoding, with subcellular resolution (the distribution of the transcript within the cell can be seen).
- "Targeted" approach
- Use of pre-designed or custom probe panels targeting hundreds/thousands of genes.
- Presence of "disease/organ-focused" panels (e.g. oncology, immune, neuro, etc.) with a set of highly informative core genes expandable through customization
- Compatibility with FFPE and frozen tissues (human and murine)
- Optical resolution of at least 200nm
- Image format compatible with the software of the instrument manufacturer or third-party software with a volume not exceeding 200G
The purchased instrumentation must also allow:
- Analysis of fixed and paraffin-embedded tissues (FFPE) and fresh frozen tissues in isopentane preserved in OCT (Fresh Frozen) or dozens of Tissue Micro Array (TMA);
- Processes up to two slides in parallel;
- Scanning an area of 12 x 24 mm per slide;
- Image acquisition through a system utilizing 4 light sources;
- Cellular segmentation through 4 acquisition modes: Membrane Proteins, Cytoplasmic Proteins, Ribosomal RNA, Nuclei;
- Optical resolution: 200 nm, extendable to 30 nm thanks to image deconvolution software;
- The use of tissue-specific panels completely validated by the supplier for the detection of RNA and proteins;
- Non-destructive tissue workflow and fully compatible with downstream tissue analysis methodologies most commonly used in research laboratories;
- Use of circularizing probes to detect mRNA targets of interest;
- Use of probes amplification steps to increase the signal/noise ratio and make the analysis more sensitive;
- Tissue-specific pre-validated panels by the supplier for RNA detection allowing the analysis of up to 380 genes in parallel;
- Multi-tissue panels pre-validated by the supplier for RNA detection allowing the analysis of more than 5000 genes in parallel;
- Customization of pre-designed panels by adding up to 100 genes or creating completely custom panels up to 480 genes;
- Analyze up to 27 proteins using a pre-designed panel;
- Use of software for analysis and visualization of results (open-source and provided free of charge);
- Reduced data sizes not exceeding 200 GB;
- Usability of acquired data without the need to be transferred to an external Cloud, remaining fully compliant with privacy laws (GDPR) in the case of analysis of human samples