The subject of the order is the equipment for the Advanced Techniques of High-Resolution Microscopy Position, being created within the framework of the High-Resolution Imaging Laboratory in Translational Research. The order has been divided into the following parts: A. Research system based on ultra-fast, super-resolution, and high-throughput confocal scanning microscopy containing three modules: 1. Part No. 1 - Super-resolution imaging module based on the stimulated emission depletion method. 2. Part No. 2 - Ultra-fast imaging module with enhanced resolution. 3. Part No. 3 - Ultra-fast high-throughput imaging module. B. Part No. 4 - Research system based on a high-resolution confocal microscope. C. Part No. 5 - Research system based on a lattice light sheet microscope. D. Part No. 6 - Research system based on a multiphoton microscope with an acousto-optical scanner. E. Part No. 7 - Research system based on a specimen scanner with confocal imaging. The contractor may submit a partial offer for any selected part/task of the order, for any selected parts/tasks of the order, or for all parts/tasks of the order. The contracting authority does not impose restrictions in this regard. Each part/task of the order is autonomous. The contracting authority will apply the relevant provisions of the Public Procurement Law separately for each specified part/task listed in this procedure, i.e., actions related to exclusion from the procedure, rejection of the offer, selection of the most advantageous offer, or annulment of the procedure are performed separately for each part/task. A separate agreement will be concluded for each part/task.
LOT-0001
Part No. 1 - Super-resolution imaging module based on the stimulated emission depletion method.
Part No. 1 - Super-resolution imaging module based on the stimulated emission depletion method..
The subject of the order is a brand new super-resolution imaging module based on the stimulated emission depletion method, which is a component of the research system based on ultra-fast, super-resolution, and high-throughput confocal scanning microscopy. The device allows super-resolution imaging (i.e., resolution better than that limited by the diffraction of light) of both fixed and live biological samples, is equipped with a confocal scanner, high-sensitivity detectors, a multi-element detector consisting of several point detectors, and a module for time-resolved measurements. The device must provide imaging with a resolution better than that limited by the diffraction limit at least using: - Deconvolution methods applied to the obtained images available in the offered software; - Optical technique of fluorescence depletion at the borders of the excited area (also known as STimulated Emission Depletion (STED)) allowing for improvement of resolution at least in the xy plane. Description of the imaging module: Detailed parameters for part 1 are specified in Appendix 2.1 to the Terms of Reference - Parameters for part 1.
LOT-0006
Part No. 6 - Research system based on a multiphoton microscope with an acousto-optical scanner.
Part No. 6 - Research system based on a multiphoton microscope with an acousto-optical scanner..
The subject of the order is a brand new, fluorescence mult photon microscope with an acousto-optical scanner for imaging biological samples and animals, equipped with an acousto-optical confocal scanner, high-sensitivity detectors, and supporting equipment for placing and immobilizing animals on the measurement stage. Description of the microscope: Detailed parameters for part 6 are specified in Appendix 2.5 to the Terms of Reference – Parameters for part 6.
LOT-0002
Part No. 2 - Ultra-fast imaging module with enhanced resolution.
Part No. 2 - Ultra-fast imaging module with enhanced resolution..
The subject of the order is a brand new ultra-fast imaging module with enhanced resolution (i.e., resolution better than that limited by the diffraction of light) of both fixed and live biological samples, which is a component of the research system based on ultra-fast, super-resolution, and high-throughput confocal scanning microscopy. The module is equipped with a confocal scanner, high-sensitivity detectors, a multi-element detector consisting of several point detectors, allowing for rapid imaging at a speed of at least 425 frames per second in a format of at least 512 by 16 pixels. The device must provide imaging with a resolution better than that limited by the diffraction limit at least using: - Deconvolution methods applied to the obtained images available in the offered software; - Methods based on image scanning microscopy (IMS) using a multi-element detector allowing for measurement of a small image consisting of at least a dozen pixels at each scanning position. Description of the imaging module: Detailed parameters for part 2 are specified in Appendix 2.2 to the Terms of Reference - Parameters for part 2.
LOT-0003
Part No. 3 - Ultra-fast high-throughput imaging module.
Part No. 3 - Ultra-fast high-throughput imaging module..
The subject of the order is a brand new, integrated module for ultra-fast high-throughput imaging, which is a component of the microscopy system for ultra-fast, super-resolution, and high-throughput confocal scanning microscopy. It allows analysis of biological models in two dimensions (2D) and three dimensions (3D), with the possibility of imaging both fixed and live biological samples, with automatic image analysis and transformation of imaging data into mathematical data, enabling simultaneous analysis of several parameters (High Content Screening (HCS)). Description of the imaging module: Detailed parameters for part 3 are specified in Appendix 2.3 to the Terms of Reference - Parameters for part 3.
LOT-0004
Part No. 4 - Research system based on a high-resolution confocal microscope.
Part No. 4 - Research system based on a high-resolution confocal microscope..
The subject of the order is a brand new, fluorescence high-resolution confocal microscope with a rotating disk allowing for examination of fixed biological samples and live observations of specimens, equipped with a rotating optical disk capable of measurements with a resolution below the limit defined by light diffraction and equipped with high-sensitivity detectors. Description of the microscope: Detailed parameters for part 4 are specified in Appendix 2.4 to the Terms of Reference - Parameters for part 4.
LOT-0005
Part No. 5 - Research system based on a lattice light sheet microscope.
Part No. 5 - Research system based on a lattice light sheet microscope..
The subject of the order is a brand new, fluorescence lattice light sheet microscope for three-dimensional (3D) imaging of live samples such as cells, spheroids, organoids, or developing organisms, allowing for fluorescence excitation using 'light sheet' illumination with the possibility of structuring light, equipped with high-sensitivity detectors and allowing for prolonged imaging lasting several hours, providing conditions of low photo-toxicity of imaging and low photo-bleaching of the imaged sample. Description of the microscope: Detailed parameters for part 5 are specified in the attached table Parameters for part 5.
LOT-0007
Part No. 7 - Research system based on a specimen scanner with confocal imaging.
Part No. 7 - Research system based on a specimen scanner with confocal imaging..
The subject of the order is a brand new specimen scanner using confocal microscopy enabling imaging of at least 100 specimens with standard sizes of 26 × 76 mm. The device should be equipped with an automated feeder, allowing for imaging of specimens dyed using histochemical methods, such as hematoxylin staining, as well as specimens dyed using immunofluorescence methods. The scanner should be adapted for imaging using bright field, dark field, polarization, phase contrast, and fluorescence techniques. The device must also allow for optical sectioning in fluorescence registration mode from only the selected focal plane of the specimen, which enables the elimination of signals outside the focal plane. The device must allow scanning of an area sized 15 × 15 mm in four fluorescence channels, using a 20x magnification objective, in no longer than 9 minutes. Additionally, it should allow scanning of an area sized 15 × 15 mm in transmitted light, using a 20x magnification objective, in no longer than 2 minutes. Description of the scanner parameters: Detailed parameters for part 7 are specified in Appendix 2.7 to the Terms of Reference - Parameters for part 7.