The subject of the order is the equipment for the Advanced Techniques of High-Resolution Microscopy Position, being created within the High-Resolution Imaging Laboratory in Translational Research. The order has been divided into the following parts: A. Research system based on ultra-fast, super-resolution, and high-throughput confocal scanning microscopy consisting of three modules: 1. Part No. 1 - Super-resolution imaging module based on the stimulated emission depletion method. 2. Part No. 2 - Ultra-fast high-resolution imaging module. 3. Part No. 3 - Ultra-fast high-throughput imaging module. B. Part No. 4 - Research system based on a high-resolution confocal microscope. C. Part No. 5 - Research system based on a lattice light sheet microscope. D. Part No. 6 - Research system based on a multi-photon microscope with an acousto-optic scanner. E. Part No. 7 - Research system based on a sample scanner with confocal imaging. The contractor may submit a partial offer for any selected part/task of the order, for any selected parts/tasks of the order, or for all parts/tasks of the order. The contracting authority does not impose any restrictions in this regard. Each part/task of the order is autonomous. The contracting authority will apply the relevant provisions of the Pzp Act separately for each of the specified parts/tasks in this procedure, i.e., actions related, among others, to exclusion from the procedure, rejection of an offer, selection of the most advantageous offer, or annulment of the procedure, are carried out separately for each part/task. A separate contract will be concluded for each part/task.

LOT-0001
Part No. 1 - Super-resolution imaging module based on the stimulated emission depletion method.
Part No. 1 - Super-resolution imaging module based on the stimulated emission depletion method..
The subject of the order is a brand new super-resolution imaging module based on the stimulated emission depletion method, constituting an element of the research system based on ultra-fast, super-resolution, and high-throughput confocal scanning microscopy. The device allows imaging in super-resolution (i.e., resolution better than that dictated by the limit resulting from the diffraction of light) of biological samples, both fixed and live, and is equipped with a confocal scanner, highly sensitive detectors, a multi-element detector consisting of many point detectors, and a module for time-resolved measurements. The device must provide imaging in resolution better than that dictated by the diffraction limit, at least using: - Deconvolution methods of obtained images available in the offered software; - Optical method for depleting fluorescence at the edges of the excited area (STimulated Emission Depletion (STED)) allowing for resolution improvement of at least in the xy plane. Module Imaging Description: Detailed parameters for part 1 are specified in Appendix 2.1 to the SWZ - Parameters for part 1.

LOT-0006
Part No. 6 - Research system based on a multi-photon microscope with an acousto-optic scanner.
Part No. 6 - Research system based on a multi-photon microscope with an acousto-optic scanner..
The subject of the order is a brand new, fluorescent multi-photon microscope with an acousto-optic scanner for imaging biological samples and animals, equipped with a confocal acousto-optic scanner, highly sensitive detectors, and auxiliary equipment to place and immobilize animals on the measurement table. Microscope Description: Detailed parameters for part 6 are specified in Appendix No. 2.6 to the SWZ – Parameters for part 6.

LOT-0002
Part No. 2 - Ultra-fast high-resolution imaging module.
Part No. 2 - Ultra-fast high-resolution imaging module..
The subject of the order is a brand new ultra-fast high-resolution imaging module (i.e., resolution better than that dictated by the limit resulting from the diffraction of light) for biological samples, both fixed and live, constituting an element of the research system based on ultra-fast, super-resolution, and high-throughput confocal scanning microscopy. The module is equipped with a confocal scanner, highly sensitive detectors, a multi-element detector consisting of many point detectors, allowing for fast imaging at a speed of at least 425 frames per second in a format of at least 512 by 16 pixels. The device must guarantee imaging in resolution better than that dictated by the diffraction limit, at least employing: - Deconvolution methods of obtained images available in the offered software - Methods based on image scanning microscopy techniques (Image Scanning Microscopy, IMS) using a multi-element detector allowing for measuring a small image comprising at least several pixels in each scanner position. Module Imaging Description: Detailed parameters for part 2 are specified in Appendix No. 2.2 to the SWZ - Parameters for part 2.

LOT-0003
Part No. 3 - Ultra-fast high-throughput imaging module.
Part No. 3 - Ultra-fast high-throughput imaging module..
The subject of the order is a brand new, integrated module for ultra-fast high-throughput imaging, constituting an element of the system for ultra-fast, super-resolution and high-throughput confocal scanning microscopy. It allows for analyses of biological models in two dimensions (2D) and three dimensions (3D), with the capability of imaging biological samples, both fixed and live, with automatic image analysis and transformation of imaging data into mathematical data, allowing for simultaneous analysis of many parameters (High Content Screening (HCS). Module Imaging Description: Detailed parameters for part 3 are specified in Appendix No. 2.3 to the SWZ - Parameters for part 3.

LOT-0004
Part No. 4 - Research system based on a high-resolution confocal microscope.
Part No. 4 - Research system based on a high-resolution confocal microscope..
The subject of the order is a brand new, fluorescent high-resolution confocal microscope with a spinning disk allowing for examining biological samples, both fixed and live observations of preparations, equipped with an optical spinning disk enabling measurements with resolution below the limit defined by the diffraction of light and equipped with highly sensitive detectors. Microscope Description: Detailed parameters for part 4 are specified in Appendix No. 2.4 to the SWZ - Parameters for part 4.

LOT-0005
Part No. 5 - Research system based on a lattice light sheet microscope.
Part No. 5 - Research system based on a lattice light sheet microscope..
The subject of the order is a brand new, fluorescent lattice light sheet microscope for three-dimensional (3D) imaging of live samples, such as cells, spheroids, organoids, or developing organisms, allowing for fluorescence excitation using 'light sheet' illumination with the possibility of excitation using structured light, equipped with highly sensitive detectors and allowing for prolonged imaging lasting several hours, ensuring low phototoxicity imaging conditions and low photobleaching of the imaged sample. Microscope Description: Detailed parameters for part 5 are specified in the table attached to the application for part 5.

LOT-0007
Part No. 7 - Research system based on a sample scanner with confocal imaging.
Part No. 7 - Research system based on a sample scanner with confocal imaging..
The subject of the order is a brand new sample scanner utilizing confocal microscopy enabling imaging of at least 100 samples of standard sizes 26 × 76 mm. The device should be equipped with an automated feeder, enable imaging of samples stained by histochemical methods, such as hematoxylin staining, as well as samples stained by immunofluorescence methods. The scanner should be suitable for imaging using bright field, dark field, polarization, phase contrast, and fluorescence techniques. The ability to perform optical sectioning in fluorescence recording mode exclusively from the selected plane of focus of the sample is also required, allowing for the elimination of signals from outside the focus plane. The device must allow scanning an area of size 15 × 15 mm in four fluorescence channels, using a 20x magnification objective, in a time not exceeding 9 minutes. Furthermore, it should allow scanning an area of size 15 × 15 mm in transmitted light, using a 20x magnification objective, in a time not exceeding 2 minutes. Scanner Parameters Description: Detailed parameters for part 7 are specified in Appendix No. 2.7 to the SWZ - Parameters for part 7.