1. The subject of the order is the delivery of a nucleic acid sequencing system using long-read technology; enabling the analysis of the structure and function of the plant holobiont - acronym [SEKWENATOR] for the B. Dobrzański Institute of Agrophysics, Polish Academy of Sciences in Lublin.
2. The scope of the order includes delivery to the Ordering Party: a nucleic acid sequencing system using long-read technology; enabling the analysis of the structure and function of the plant holobiont - acronym [SEKWENATOR].
The Ordering Party requires the offering of a warranty period for the subject of the order for a minimum of 12 months. Offers with a shorter warranty period than specified above will be rejected. Attention! The warranty period is a criterion for evaluating offers according to Chapter XVIII of the SWZ.
3. The name of the order included in the title does not constitute a proprietary name of the manufacturers/distributors of the products, but is a purely auxiliary designation applied by the Ordering Party.
4. A detailed description of the subject of the order is included in Appendix No. 1 to the SWZ.
LOT-0001
A-2401-10/2026
Delivery of a nucleic acid sequencing system using long-read technology; enabling the analysis of the structure and function of the plant holobiont - acronym [SEKWENATOR] for the B. Dobrzański Institute of Agrophysics, Polish Academy of Sciences in Lublin.
The subject of the order is the delivery of a nucleic acid sequencing system using long-read technology; enabling the analysis of the structure and function of the plant holobiont - acronym [SEKWENATOR] for the B. Dobrzański Institute of Agrophysics, Polish Academy of Sciences in Lublin.
The scope of the order includes delivery to the Ordering Party: a nucleic acid sequencing system using long-read technology; enabling the analysis of the structure and function of the plant holobiont - acronym [SEKWENATOR]. A detailed description of the subject of the order is included in Appendix No. 1 to the SWZ.
MINIMUM REQUIRED TECHNICAL AND OPERATIONAL PARAMETERS
The nucleic acid sequencing system using long-read technology; enabling the analysis of the structure and function of the plant holobiont - acronym [SEKWENATOR]
No. MINIMUM REQUIRED PARAMETER: CONDITION: OFFERED PARAMETERS/CONDITIONS/MODEL (filled by the contractor):
1. Nucleic acid sequencing system using long-read technology; enabling the analysis of the structure and function of the plant holobiont - acronym [SEKWENATOR] Mandatory condition
2. Device weight not greater than 360 kg Mandatory condition
3. Work area occupied by the device not greater than 0.6 m2 Mandatory condition
4. Device dimensions not greater than: height 170 cm, width 95 cm, depth 90 cm Mandatory condition
5. Device equipped with an LCD display with a touch panel for interactive user operation Mandatory condition
6. Work space of the device equipped with: robotic arm, space for reagent plates, space for tip containers, space for sequencing plates (chips), waste disposal area Mandatory condition
7. Device enabling single DNA molecule sequencing in real-time Mandatory condition
8. Device providing the ability to obtain >5Gb of data and >370,000 reads from one chip, >80Gb of data and >6,000,000 reads at full device throughput Mandatory condition
9. DNA sequencing using deoxyribonucleotide triphosphates covalently linked to appropriate fluorophores – fluorophores attached to 5’ terminal phosphate residue (phosphate at the gamma position) Mandatory condition
10. Sequencing reactions occur in reaction wells where a single molecule of recombinant DNA polymerase and DNA/cDNA template is immobilized Mandatory condition
11. Sequencing reactions of single DNA molecules in real-time occur in volumes on the order of zeptoliters Mandatory condition
12. Genomic DNA sequencing without the need for prior amplification of biological material by polymerase chain reaction – PCR Mandatory condition
13. Device enabling full-length transcript sequencing after prior conversion of RNA material to complementary DNA (cDNA) using reverse transcription method Mandatory condition
14. Device enabling positioning of epigenetic modifications in the genome solely through analysis of the kinetics of DNA sequencing reactions Mandatory condition
15. Device allowing the positioning of epigenetic modifications in the DNA sequence Mandatory condition
16. The ability to position the 5mC epigenetic modification without the need for prior conversion using sodium bisulfite Mandatory condition
17. Device enabling the obtaining of single reads of nucleic acid sequences of DNA molecules averaging no less than 20,000 bp Mandatory condition
18. Device enabling the obtaining of nucleic acid sequence reads longer than 80 bp Mandatory condition
19. Information about the nucleotide sequences obtained in the sequencing reaction enabling structural analysis of the genome considering: VNTR, duplications, dispersed duplications, translocations, inversions, insertions, and single nucleotide polymorphisms (SNPs). Mandatory condition
20. Device operating in a technology enabling sequencing and positioning of long repeat regions in the genome rich in CGG trinucleotide and regions rich in AT residues Mandatory condition
21. Device operating in a technology enabling sequencing of DNA homopolymers and providing information on their length – for example, characteristics of polyA tail lengths present in mRNA transcripts Mandatory condition
22. Device delivered with appropriate plates (chips) enabling simultaneous real-time sequencing of single DNA/cDNA molecules in reaction wells, with a quantity of 1,000,000 reaction wells per plate Mandatory condition
23. Device delivered with a dedicated UPS system Mandatory condition
24. Weight of the UPS device not greater than 60 kg Mandatory condition
25. Dimensions of the UPS device not greater than: height 75 cm, width 20 cm, depth 60 cm Mandatory condition
26. Device delivered with a set for constructing DNA libraries – DNA molecules serving as templates for sequencing reactions are circular in form due to the use of universal adapters with a ‘hairpin’ structure (stem-loop structure) containing:
a) Automatic station for preparing DNA libraries:
a. equipped with interchangeable modules, at least:
i. thermocycler
ii. heating and cooling device
iii. thermal shaker
iv. magnetic rack
v. at least one rack feeder with tips
vi. transport arm for plates
vii. exchangeable pipettes – at least one 96-channel with a working volume range of 5-1000 µl and at least two 8-channel pipettes with working volumes of 1-50 µl and 5-1000 µl
viii. waste disposal for used tips
(...) continued in section 5.1.6 General Information - Additional Information