Electronic International Public Open Competition (systemic number 359745 for the Supply of Pathological Anatomy Laboratory Reagents for the needs of the University General Hospital of Larissa

LOT-0001
1.
100bp LADDER, molecular size DNA marker ranging from 100-1500bp. Must display 15 bands in electrophoresis differing by 100bp (concentration 1μg/μl) in a package of 50μg.
100bp LADDER, molecular size DNA marker ranging from 100-1500bp. Must display 15 bands in electrophoresis differing by 100bp (concentration 1μg/μl) in a package of 50μg

LOT-0002
2.
100mM dNTP Set, Kit of deoxynucleotides d-ATP, d-GTP, d-CTP, d-TTP (nucleotide solutions) 100mM d-NTP’sset, 25mM each.
100mM dNTP Set, Kit of deoxynucleotides d-ATP, d-GTP, d-CTP, d-TTP (nucleotide solutions) 100mM d-NTP’sset, 25mM each

LOT-0003
3.
HOT START POLYMERASE. Polymerase bound with a mixture of specific monoclonal antibodies that inhibit its action. The enzyme must activate during the initial denaturation of PCR (automatic HOTSTART) and allow the reaction to be set up at room temperature. Must have a concentration of 5u/ml and come with an appropriate 10X buffer solution and a separate MgCl2 solution (package for at least 600 reactions).
1HOT START POLYMERASE. Polymerase bound with a mixture of specific monoclonal antibodies that inhibit its action. The enzyme must activate during the initial denaturation of PCR (automatic HOTSTART) and allow the reaction to be set up at room temperature. Must have a concentration of 5u/ml and come with an appropriate 10X buffer solution and a separate MgCl2 solution (package for at least 600 reactions/kit)

LOT-0004
4.
PHOSPHATE BUFFERED SALINE (PBS) 7.4 (1X) LIQUID, in a package of 1LT.
PHOSPHATE BUFFERED SALINE (PBS) 7.4 (1X) LIQUID, in a package of 1LT

LOT-0005
5.
ULTRA PURE Agarose for separating fragments 100bp-30Kb, in a plastic flexible bag package with a special spout for easy handling. Package of at least 500 grams..
ULTRA PURE Agarose for separating fragments 100bp-30Kb, in a plastic flexible bag package with a special spout for easy handling. Package of at least 500 grams.

LOT-0006
6.
Complete KIT for DNA purification from agarose or polyacrylamide gel, with spin-columns, for the recovery of DNA fragments of 70bp-10kb. KIT of at least 50 reactions .
Complete KIT for DNA purification from agarose or polyacrylamide gel, with spin-columns, for the recovery of DNA fragments of 70bp-10kb. KIT of at least 50 reactions

LOT-0007
7.
Complete HER2/CEN17 Dual Color CISH kit for the detection of amplification of the HER2/ERBB2 gene in solid neoplasms, suitable for paraffin sections containing all necessary reagents and solutions in quantities sufficient for at least 40 tests/kit. Must be suitable for in vitro diagnostic use & necessarily evaluable in external quality control programs with satisfactory results.
Complete HER2/CEN17 Dual Color CISH kit for the detection of amplification of the HER2/ERBB2 gene in solid neoplasms, suitable for paraffin sections containing all necessary reagents and solutions in quantities sufficient for at least 40 tests/kit. Must be suitable for in vitro diagnostic use & necessarily evaluable in external quality control programs with satisfactory results (to be submitted).

LOT-0008
8.
kit for the detection of mutations of the C-Kit and PDGFR genes in patients with gastrointestinal cancer (GIST) using the realtime PCR technique. Must detect mutations in exons 9, 11, 13, 17 of the c-Kit gene and in exon 18 of the PDGFR gene. Must be suitable for application to fresh frozen tissue samples and paraffin sections (FFPE). .
kit for the detection of mutations of the C-Kit and PDGFR genes in patients with gastrointestinal cancer (GIST) using the realtime PCR technique. Must detect mutations in exons 9, 11, 13, 17 of the c-Kit gene and in exon 18 of the PDGFR gene. Must be suitable for application to fresh frozen tissue samples and paraffin sections (FFPE). The package must include reagents sufficient for at least 40 mutation detection reactions. The kit must use polymerase reaction and specific probes against alleles for the presence of mutations. Must have high sensitivity and detect mutations at levels of 1%. The examination must take about 2 hours from DNA to result. The kit must bear CE/IVD marking.

LOT-0009
9.
Her2 4B5 RTU.
Her2 4B5 RTU

LOT-0010
10.
MSH-6 SP93 RTU.
MSH-6 SP93 RTU

LOT-0011
11.
MLH-1 M1 RTU.
MLH-1 M1 RTU

LOT-0013
12.
MSH-2 G219-1129 RTU.
MSH-2 G219-1129 RTU

LOT-0014
13.
PMS2 A16-4 RTU.
PMS2 A16-4 RTU

LOT-0015
14.
HERCEPT test .
HERCEPT test

LOT-0016
15.
COMPLETE SET OF REAGENTS FOR THE DIAGNOSTIC TEST OF PDL-1 (22C3), MUST INCLUDE ALL NECESSARY REAGENTS AND POSITIVE CONTROLS FOR THE ANTIGEN TEST IN THE FRAMEWORK OF PHARMACEUTICAL TREATMENT WITH KEYTRUDA (pembrolizumab) IN PATIENTS WITH NSCLC. MUST HAVE CE/IVD AND FDA APPROVAL. MUST BE SUFFICIENT FOR AT LEAST 50 TESTS .
COMPLETE SET OF REAGENTS FOR THE DIAGNOSTIC TEST OF PDL-1 (22C3), MUST INCLUDE ALL NECESSARY REAGENTS AND POSITIVE CONTROLS FOR THE ANTIGEN TEST IN THE FRAMEWORK OF PHARMACEUTICAL TREATMENT WITH KEYTRUDA (pembrolizumab) IN PATIENTS WITH NSCLC. MUST HAVE CE/IVD AND FDA APPROVAL. MUST BE SUFFICIENT FOR AT LEAST 50 TESTS

LOT-0017
16.
ERG CLONE clone EP111 RTU 7ml.
ERG CLONE clone EP111 RTU 7ml