Acquisition of a high-throughput sequencing platform based on nanopore technology including warranty/maintenance services for 24 months.
The equipment must meet the following characteristics:
nanopore technology-based sequencing platform for the direct sequencing of native DNA and RNA molecules through long reads (long-read);
the ability to perform sequencing without the need for PCR amplification, DNA synthesis, or optical detection;
direct identification of epigenetic modifications of DNA and RNA during the sequencing process;
high sequencing capacity, suitable for large-scale genomic and transcriptomic applications;
real-time data acquisition, basecalling, and processing, with immediate availability of results during the experiment execution;
the possibility to simultaneously perform independent experiments through dedicated and autonomously manageable flow cells;
the ability to stop sequencing upon reaching the desired coverage and to recover the library for subsequent reuse;
the capability to perform selective enrichment or depletion of specific genomic regions directly during sequencing (Adaptive Sampling), without additional library preparation steps, PCR amplification, or chemical treatments;
integrated system comprising instrumentation control, data acquisition, basecalling, primary bioinformatics analyses, and sequencing workflow management;
high-throughput platform characterized by a compact footprint relative to performance, allowing installation in research laboratories without the need for dedicated infrastructure.
The equipment to be purchased must also:
enable obtaining long reads (long-read), suitable for advanced genomic, transcriptomic, and epigenomic applications;
integrate data acquisition, basecalling, and primary bioinformatics analyses in real time, also allowing direct alignment of sequences to reference genomes during the experiment execution;
enable the simultaneous execution of up to 24 fully independent experiments via 24 dedicated flow cells, each manageable independently, allowing start, interruption, replacement, or reuse of individual flow cells without interfering with other ongoing experiments;
permit sequencing of native DNA and RNA molecules ranging from approximately 20 bp to millions of bases, suitable for both short fragment analysis and ultra-long molecule applications;
guarantee a production capacity of up to 200 Gb of data per single flow cell, enabling about 30× coverage of the human genome per flow cell and suitable for high complexity genomic and transcriptomic applications.
LOT-0001
NOTICE OF PRELIMINARY MARKET CONSULTATION PREPARATORY TO THE INITIATION OF A NEGOTIATED PROCEDURE WITHOUT PUBLICATION OF A CONTRACT NOTICE PURSUANT TO ART. 76 PARAGRAPH 2 LETTER B), POINT 2 OF LEGISLATIVE DECREE 36/2023 FOR THE PROCUREMENT OF A HIGH-THROUGHPUT SEQUENCING PLATFORM BASED ON NANOPORE TECHNOLOGY INCLUDING WARRANTY/MAINTENANCE SERVICES FOR 24 MONTHS.
CUP B53C2500370000.
Acquisition of a high-throughput sequencing platform based on nanopore technology including warranty/maintenance services for 24 months.
The equipment must meet the following characteristics:
nanopore technology-based sequencing platform for the direct sequencing of native DNA and RNA molecules through long reads (long-read);
the ability to perform sequencing without the need for PCR amplification, DNA synthesis, or optical detection;
direct identification of epigenetic modifications of DNA and RNA during the sequencing process;
high sequencing capacity, suitable for large-scale genomic and transcriptomic applications;
real-time data acquisition, basecalling, and processing, with immediate availability of results during the experiment execution;
the possibility to simultaneously perform independent experiments through dedicated and autonomously manageable flow cells;
the ability to stop sequencing upon reaching the desired coverage and to recover the library for subsequent reuse;
the capability to perform selective enrichment or depletion of specific genomic regions directly during sequencing (Adaptive Sampling), without additional library preparation steps, PCR amplification, or chemical treatments;
integrated system comprising instrumentation control, data acquisition, basecalling, primary bioinformatics analyses, and sequencing workflow management;
high-throughput platform characterized by a compact footprint relative to performance, allowing installation in research laboratories without the need for dedicated infrastructure.
The equipment to be purchased must also:
enable obtaining long reads (long-read), suitable for advanced genomic, transcriptomic, and epigenomic applications;
integrate data acquisition, basecalling, and primary bioinformatics analyses in real time, also allowing direct alignment of sequences to reference genomes during the experiment execution;
enable the simultaneous execution of up to 24 fully independent experiments via 24 dedicated flow cells, each manageable independently, allowing start, interruption, replacement, or reuse of individual flow cells without interfering with other ongoing experiments;
permit sequencing of native DNA and RNA molecules ranging from approximately 20 bp to millions of bases, suitable for both short fragment analysis and ultra-long molecule applications;
guarantee a production capacity of up to 200 Gb of data per single flow cell, enabling about 30× coverage of the human genome per flow cell and suitable for high complexity genomic and transcriptomic applications.